{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T03:14:09Z","timestamp":1788318849816,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2025,7,20]],"date-time":"2025-07-20T00:00:00Z","timestamp":1752969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Saururus chinensis<\/jats:italic> has traditionally been used to treat various diseases. Biologically active compounds isolated from <jats:italic>S. chinensis<\/jats:italic> exhibit diverse pharmacological activities. The aim of this study was to investigate the antiosteosarcoma effects of saucerneol (Sauc), a lignan, purified from the aerial parts of <jats:italic>S. chinensis<\/jats:italic> using in vitro and ex vivo experimental models. Sauc was purified from the methanolic extract of <jats:italic>S. chinensis<\/jats:italic>. It exhibited toxicity against MG63 and SJSA\u20101 cells (human osteosarcoma cell lines), inducing apoptotic morphological changes and suppressing cell migration. Sauc triggered PARP cleavage and decreased the expression of antiapoptotic proteins. It also disrupted mitochondrial membrane potential and increased reactive oxygen species (ROS) generation. Proteome profiling, western blotting, and immunocytochemistry revealed that Sauc inhibited the JAK2\/STAT3 pathway. Furthermore, Sauc downregulated the expression of metastasis\u2010associated proteins, suppressed invasion through extracellular matrix\u2010coated membranes, and inhibited anchorage\u2010independent cell growth. In an ex vivo bone organ culture, Sauc attenuated tumor\u2010induced osteolysis. This study demonstrated that Sauc exerts anti\u2010osteosarcoma effects by inducing apoptosis, inhibiting cell migration and invasion in vitro, and mitigating metastasis\u2010associated bone degradation ex vivo. Thus, Sauc holds promise as a protective compound in daily health supplements and a therapeutic agent against human osteosarcoma.<\/jats:p>","DOI":"10.1002\/mnfr.70187","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T06:21:34Z","timestamp":1753078894000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Saucerneol Inhibits the Growth, Migration, and Invasion of Osteosarcoma Cells In Vitro and Prevents Metastasis\u2010Associated Osteolysis Ex Vivo"],"prefix":"10.1002","volume":"69","author":[{"given":"Hyung\u2010Mun","family":"Yun","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology School of Dentistry Kyung Hee University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Xuan","family":"Nhiem","sequence":"additional","affiliation":[{"name":"Institute of Marine Biochemistry Vietnam Academy of 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