{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T15:35:16Z","timestamp":1765208116207,"version":"3.46.0"},"reference-count":27,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study investigated the anti\u2010inflammatory and skin barrier\u2010restoring effects of a 50% ethanol extract of\n                    <jats:italic>Rosa rugosa<\/jats:italic>\n                    Thunb. (RT50) using both in vitro and in vivo models, including HaCaT keratinocytes and a 1\u2010Chloro\u20102,4\u2010dinitrobenzene (DNCB)\u2010induced ear edema mouse model. The aim was to evaluate the potential efficacy of RT50 in treating hypersensitivity\u2010related skin conditions, such as atopic and contact dermatitis, and to explore natural therapeutic alternatives with fewer side effects for managing inflammatory skin diseases. Cytotoxicity testing identified non\u2010toxic concentrations of 10, 30, and 60\u00a0\u00b5g\/mL for further analysis. RT50 significantly reduced tumor necrosis factor\u2010\u03b1\/interferon\u2010\u03b3\u2010induced pro\u2010inflammatory cytokines and chemokines in HaCaT cells, demonstrating its anti\u2010inflammatory properties. In vivo, treatment with RT50 significantly reduced DNCB\u2010induced ear edema, as confirmed by histological analysis. Epidermal and dermal thickness and mast cell infiltration were reduced in a concentration\u2010dependent manner. Serum analysis showed that RT50 decreased elevated levels of Immunoglobulin E\/Immunoglobulin G caused by DNCB exposure. The gene expression results further showed that RT50 suppressed DNCB\u2010induced pro\u2010inflammatory cytokines and chemokines in ear tissues. Additionally, RT50 restored skin barrier integrity, which was disrupted by DNCB. Key skin barrier proteins, including filaggrin, involucrin, and loricrin, showed increased expression after RT50 treatment. Factors associated with tight junctions were also positively affected, suggesting that RT50 may play a key role in repairing skin barrier function. These findings highlight RT50 as a promising therapeutic candidate for addressing inflammatory skin conditions by both reducing inflammation and restoring skin barrier integrity.\n                  <\/jats:p>","DOI":"10.1002\/mnfr.70214","type":"journal-article","created":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T07:32:17Z","timestamp":1755675137000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effectiveness of Ethanol Extract of\n                    <i>Rosa rugosa<\/i>\n                    Thunb. on Ear Edema"],"prefix":"10.1002","volume":"69","author":[{"given":"Seon Gyeong","family":"Bak","sequence":"first","affiliation":[{"name":"Functional Biomaterial Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Jeongeup South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nisansala","family":"Chandimali","sequence":"additional","affiliation":[{"name":"Functional Biomaterial Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Jeongeup South Korea"},{"name":"Applied Biological Engineering KRIBB School of Biotechnology University of Science and Technology  Daejeon South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun Jae","family":"Park","sequence":"additional","affiliation":[{"name":"Functional Biomaterial Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Jeongeup South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1908-7666","authenticated-orcid":false,"given":"Seung Woong","family":"Lee","sequence":"additional","affiliation":[{"name":"Functional Biomaterial Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Jeongeup South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaehoon","family":"Bae","sequence":"additional","affiliation":[{"name":"Functional Food Research Institute Industry\u2010University Cooperation Foundation Daegu Hanny University  Gyeongsan South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mun\u2010Chual","family":"Rho","sequence":"additional","affiliation":[{"name":"Functional Biomaterial Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Jeongeup South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung Jae","family":"Lee","sequence":"additional","affiliation":[{"name":"Functional Biomaterial Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)  Jeongeup South Korea"},{"name":"Applied Biological Engineering KRIBB School of Biotechnology University of Science and Technology  Daejeon South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,20]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"375","article-title":"Ultrasound\u2010Assisted Extraction and Antioxidant Activity of Phenolic and Flavonoid Compounds and Ascorbic Acid From rugosa Rose (Rosa rugosa Thunb.) Fruit","volume":"27","author":"Um M.","year":"2018","journal-title":"Food Science and Biotechnology"},{"key":"e_1_2_9_3_1","first-page":"1","article-title":"Total Flavonoids From Rosa rugosa Thunb.: A Comprehensive Review of Its Extraction and Purification Process, Chemical Composition, Biological Effect and Applications","volume":"398","author":"Dong G.","year":"2024","journal-title":"Naunyn\u2010Schmiedeberg's Archives of Pharmacology"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11418-010-0418-5"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.25135\/rnp.60.17.12.191"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2014.11.009"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.4062\/biomolther.2015.090"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy12020238"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.5658\/WOOD.2018.46.1.38"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/2854238"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2012.09.038"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants13091266"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.5010\/JPB.2022.49.2.145"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.111952"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6767"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-7020-6896-6.00044-2"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21082867"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2010.07.004"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/ddg.14709"},{"key":"e_1_2_9_20_1","first-page":"713","article-title":"Irritant and Allergic Contact Dermatitis\u2013Skin Lesion Characteristics","volume":"57","author":"Novak\u2010Bili\u0107 G.","year":"2018","journal-title":"Acta Clinica Croatica"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-018-8705-0"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/2475699"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2013.03.001"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109743"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.3610"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-4-431-99365-0_10"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2023.06.006"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21155382"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.70214","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T14:34:09Z","timestamp":1765204449000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.70214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,20]]},"references-count":27,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/mnfr.70214"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.70214","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"type":"print","value":"1613-4125"},{"type":"electronic","value":"1613-4133"}],"subject":[],"published":{"date-parts":[[2025,8,20]]},"assertion":[{"value":"2025-03-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70214"}}