{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T16:43:06Z","timestamp":1790959386645,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T00:00:00Z","timestamp":1756944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      The maternal gut microbiome is involved in adapting immune responses to the presence of the semiallogeneic foetus. We have previously shown that antibiotics\u2010induced gut dysbiosis, alterations in the maternal immune response and decreased foetal and placental weight. Here, we tested whether Bifidobacterium animalis subsp.\n                      <jats:italic>lactis<\/jats:italic>\n                      BL\u201099 (BL\u201099) could improve antibiotics\u2010induced gut dysbiosis, maternal immune responses and foetal and placental weight. To do so, pregnant mice received antibiotics in their drinking water (day 9\u201016) and BL\u201099 via oral gavage (day 9\u201018). After sacrifice (day 18) immune responses were measured using flowcytometry. BL\u201099 increased placental weight in antibiotics\u2010treated pregnant mice. BL\u201099 did not significantly change the maternal microbiome, but improved maternal immune responses by decreasing splenic Th1 cells and Treg cells, and increasing FoxP3\/RoR\u03b3T double\u2010positive cells in the Peyer's patches to levels observed in control pregnant mice. BL\u201099 also improved monocyte subsets and activation status. Additionally, BL\u201099 changed foetal monocyte subsets and activational status and increased foetal splenic Th cells. We thus showed that the effect of antibiotics treatment on immune cells and placental weight was mitigated by supplementation of BL\u201099. We suggest that pregnancy complications associated with a disturbed microbiome and immune responses, such as preeclampsia or obese pregnancies, could benefit from BL\u201099 supplementation. This should be tested in future studies.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mnfr.70220","type":"journal-article","created":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T13:39:21Z","timestamp":1756993161000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>Bifidobacterium animalis<\/i>\n                    subsp.\n                    <i>Lactis<\/i>\n                    BL\u201099 Improves Maternal and Fetal Immune Responses and Pregnancy Outcomes in Pregnant Antibiotics\u2010Treated Mice"],"prefix":"10.1002","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5860-3993","authenticated-orcid":false,"given":"Marijke M.","family":"Faas","sequence":"first","affiliation":[{"name":"Department of Pathology and Medical Biology University of Groningen and University Medical Center Groningen  Groningen the Netherlands"},{"name":"Department of Obstetrics and Gynaecology University of Groningen and University Medical Center Groningen  Groningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lieske","family":"Wekema","sequence":"additional","affiliation":[{"name":"Department of Pathology and Medical Biology University of Groningen and University Medical Center Groningen  Groningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolien A.","family":"van Loo\u2010Bouwman","sequence":"additional","affiliation":[{"name":"Yili Innovation Center Europe  Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisela A.","family":"Weiss","sequence":"additional","affiliation":[{"name":"Yili Innovation Center Europe  Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010lian","family":"Hung","sequence":"additional","affiliation":[{"name":"National Center of Technology Innovation For Dairy  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart J.","family":"de Haan","sequence":"additional","affiliation":[{"name":"Department of Pathology and Medical Biology University of Groningen and University Medical Center Groningen  Groningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra M.","family":"Smink","sequence":"additional","affiliation":[{"name":"Department of Pathology and Medical Biology University of Groningen and University Medical Center Groningen  Groningen the 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