{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T17:47:56Z","timestamp":1764006476109,"version":"3.45.0"},"reference-count":34,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2025,8,31]],"date-time":"2025-08-31T00:00:00Z","timestamp":1756598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","award":["ICARNF\/2020\u201025"],"award-info":[{"award-number":["ICARNF\/2020\u201025"]}],"id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Tropomyosin is a significant allergen found in invertebrates and is recognized as one of the primary triggers of food\u2010induced anaphylaxis. Though many researchers have done the characterization of allergic tropomyosin, a comparative analysis of the allergenicity of vertebrate (porcine) and invertebrate (crab) tropomyosin is still lacking. The present study aims to study the differences in IgE, tropomyosin\u2010specific IgE, and histamine levels between vertebrate (porcine) and invertebrate tropomyosin (crab) using an animal experimental model. Tropomyosin was extracted from two selected crabs, marine water (\n                    <jats:italic>Scylla olivacea<\/jats:italic>\n                    ) and freshwater (\n                    <jats:italic>Charybdis ferriatus<\/jats:italic>\n                    ). A high level of systemic anaphylactic reactions was observed in the mice groups treated with crab tropomyosin. In addition, high levels of IgE, tropomyosin\u2010specific IgE, and histamine levels as revealed by ELISA (Enzyme\u2010Linked Immunosorbent Assay) analysis. Histopathological examination exhibited changes in the liver and gastrointestinal regions of the mice groups treated with crab tropomyosin. The variations in the amounts of transcription factors like T\u2010bet, GATA\u20103, and Foxp3 were also evaluated through gene expression analysis. The presence of GATA\u20103\u2032 transcription factor was also identified in the spleen of mice treated with crab tropomyosin. This study helps for the better diagnosis and treatment of food allergies, especially shellfish allergies.\n                  <\/jats:p>","DOI":"10.1002\/mnfr.70240","type":"journal-article","created":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T05:30:12Z","timestamp":1756704612000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Extraction and Purification of Tropomyosin From Crabs\n                    <i>Scylla olivacea<\/i>\n                    and\n                    <i>Charybdis ferriatus<\/i>\n                    : Assessment of Allergenicity and Sensitizing Capacity Using Animal Experimental Model"],"prefix":"10.1002","volume":"69","author":[{"given":"Neha","family":"Omgy","sequence":"first","affiliation":[{"name":"Faculty of Ocean Science and Technology Kerala University of Fisheries and Ocean Studies  Cochin Kerala India"},{"name":"Biochemistry and Nutrition Division Indian Council of Agricultural Research\u2010Central Institute of Fisheries Technology Cochin Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rangasamy","family":"Anandan","sequence":"additional","affiliation":[{"name":"Biochemistry and Nutrition Division Indian Council of Agricultural Research\u2010Central Institute of Fisheries Technology Cochin Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sankar","family":"Thazhakot Vasunambesan","sequence":"additional","affiliation":[{"name":"Biochemistry and Nutrition Division Indian Council of Agricultural Research\u2010Central Institute of Fisheries Technology Cochin Kerala India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijay Kumar Reddy","family":"Surasani","sequence":"additional","affiliation":[{"name":"Department of Fish Processing Technology College of Fisheries Guru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celine Hilda Mary","family":"Savari Arockiam","sequence":"additional","affiliation":[{"name":"PG &amp; Research Department of Biochemistry St. Joseph College of Arts &amp; Science  Cuddalore Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manjanaik","family":"Bojayanaik","sequence":"additional","affiliation":[{"name":"Department of Fish Processing Technology College of Fisheries Karnataka Veterinary, Animal and Fisheries Sciences University Mangalore Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veena Shetty","family":"Alandur","sequence":"additional","affiliation":[{"name":"Department of Microbiology K S Hegde Medical Academy (KSHEMA) NITTE Deemed to Be University  Mangalore Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ambrose","family":"Paulraj","sequence":"additional","affiliation":[{"name":"P.G and Research Department of Zoology Government Arts College for Men  Krishnagiri Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavan Kumar","family":"Dara","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Faculty of Science and Humanities SRM Institute of Science and Technology Kattankulathur Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201500936"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-011-8284-9"},{"key":"e_1_2_9_4_1","first-page":"3","article-title":"Shellfish Allergy\u2014An Asia\u2010Pacific Perspective","volume":"30","author":"Lee A. 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