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However, the underlying mechanisms remain unknown. This study examined the effects and potential mechanisms of blueberries, blackberries, and bananas on ME using an in vitro human intestinal\u2010immune cell model. Palm oil with LPS was added to intestinal Caco\u20102 cells seeded on Transwell inserts, recapitulating dietary fat absorption. Higher levels of basolateral LPS were observed when Caco\u20102 cells were cotreated with palm oil and LPS compared to control and LPS, supporting lipid\u2010induced LPS translocation. To examine the bioactivity of translocated LPS, THP1\u2010Lucia nuclear factor kappa B (NF\u2010\u03baB) macrophages were exposed to basolateral conditioned media from Caco\u20102 cells, and NF\u2010\u03baB activation was assessed. Basolateral conditioned medium from Caco\u20102 cells cotreated with digested palm oil and LPS induced higher macrophage NF\u2010\u03baB activation compared to only palm oil. Interestingly, fruits reduced the palm oil + LPS\u2010mediated NF\u2010\u03baB activation in macrophages. Transcriptomic and protein\u2010level analyses suggest berries modulate the lipid\u2010induced LPS translocation, likely via clathrin\u2010mediated transcytosis with a minor chylomicron\u2010mediated contribution. The anti\u2010inflammatory effects of berry\u2010rich diets may be mediated by preventing ME.<\/jats:p>","DOI":"10.1002\/mnfr.70311","type":"journal-article","created":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T07:53:33Z","timestamp":1762934013000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Blueberries Reduce Palm Oil\u2010Induced Metabolic Endotoxemia in an In Vitro Human Intestinal\u2010Immune Cell Model"],"prefix":"10.1002","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2508-0740","authenticated-orcid":false,"given":"Aina","family":"Casademont\u2010Roca","sequence":"first","affiliation":[{"name":"Division of Human Nutrition and Health Wageningen University &amp; Research  Wageningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monic M. 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