{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T03:23:58Z","timestamp":1791689038490,"version":"4.3.4"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272318"],"award-info":[{"award-number":["32272318"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20211292"],"award-info":[{"award-number":["BK20211292"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Type 2 diabetes mellitus (T2DM) is characterized by impaired glucose and lipid metabolism and gut microbiota dysbiosis. This study investigated the therapeutic potential and mechanisms of pumpkin polysaccharide fraction 3 (PPS3), a pumpkin polysaccharide, in a T2DM mouse model. Results demonstrated that PPS3 administration significantly increased body weight, reduced fasting blood glucose, attenuated hyperphagia and polydipsia, and enhanced glucose tolerance and insulin sensitivity. Hepatic analyses showed that PPS3 promoted glycogen synthesis and upregulated key glycolytic enzymes, hexokinase, and pyruvate kinase, contributing to restored glucose homeostasis. Mechanistically, PPS3 inhibited p38 mitogen\u2010activated protein kinase (p38 MAPK) signaling and activated PTEN\u2010induced kinase 1 (PINK1)\u2010Parkin (PRKN)\u2010mediated mitophagy, enhancing mitochondrial quality control. Gut microbiota analysis showed that PPS3 reduced the\n                    <jats:italic>Firmicutes<\/jats:italic>\n                    \/\n                    <jats:italic>Bacteroidota<\/jats:italic>\n                    ratio and pathogenic genera while enriching beneficial bacteria including\n                    <jats:italic>Akkermansia<\/jats:italic>\n                    and short\u2010chain fatty acid (SCFA) producers. Fecal metabolomics revealed partial restoration of metabolic disturbances, notably increased levels of propionyl\u2010l\u2010carnitine, indole\u20103\u2010lactic acid, and \u03b2\u2010lapachone. PPS3 exerted multifaceted metabolic benefits via inhibition of p38 MAPK, activation of PINK1\u2010PRKN mitophagy, and gut microbiota modulation, positioning it as a promising candidate for T2DM intervention.\n                  <\/jats:p>","DOI":"10.1002\/mnfr.70343","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T09:55:07Z","timestamp":1764410107000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Pumpkin Polysaccharide Ameliorates Type 2 Diabetes in Mice via Inhibition of p38 MAPK, Activation of PINK1\u2010PRKN Mitophagy, and Gut Microbiota Modulation"],"prefix":"10.1002","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1091-3084","authenticated-orcid":false,"given":"Haizhao","family":"Song","sequence":"first","affiliation":[{"name":"College of Food Science and Engineering Nanjing University of Finance and Economics\/Collaborative Innovation Center for Modern Grain Circulation and Safety  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangmin","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Nanjing University of Finance and Economics\/Collaborative Innovation Center for Modern Grain Circulation and Safety  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Nanjing University of Finance and Economics\/Collaborative Innovation Center for Modern Grain Circulation and Safety  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5280-2967","authenticated-orcid":false,"given":"Fang","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Nanjing University of Finance and Economics\/Collaborative Innovation Center for Modern Grain Circulation and Safety  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6726-9069","authenticated-orcid":false,"given":"Luanfeng","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Nanjing University of Finance and Economics\/Collaborative Innovation Center for Modern Grain Circulation and Safety  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Xiong","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Nanjing University of Finance and Economics\/Collaborative Innovation Center for Modern Grain Circulation and Safety  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinchun","family":"Shen","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Nanjing University of Finance and Economics\/Collaborative Innovation 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