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This study evaluated whether cyanidin\u20103\u2010O\u2010glucoside (C3G, 100\u00a0mg\/kg) protects against liver injury by the representative HAA 2\u2010amino\u20103\u2010methylimidazo[4,5\u2010f]quinoline (IQ, 20\u00a0mg\/kg) in mice, and explored mechanisms by integrated approaches: biochemical assays, histology, Western blot, and molecular dynamics (MD) simulations. Results demonstrated that C3G significantly ameliorated IQ\u2010induced liver injury, as indicated by reduced serum AST and ALT and inflammatory cytokines (IL\u20106, IL\u20101\u03b2, TNF\u2010\u03b1) levels. Additionally, C3G restored redox homeostasis by inhibiting lipid peroxidation and protein carbonylation, while enhancing antioxidant defenses (GSH, GSH\u2010Px, and SOD). Mechanistically, C3G exerted its protective effects via multiple anti\u2010apoptotic pathways. Specifically, it attenuated mitochondrial apoptosis by lowering the Bax\/Bcl\u20102 ratio and inhibiting cytochrome c\u2010caspase\u20109\/3 activation. It also alleviated endoplasmic reticulum (ER) stress by downregulating GRP78\/ATF4\/CHOP and caspase\u201012. Furthermore, C3G interfered with death receptor signaling by reducing Fas\/FasL\/FADD activation and caspase\u20108, while upregulating CFLAR. MD simulations further demonstrated that C3G binds to the hydrophobic pocket of BAX and weakens BAX\u2010BIM interactions. These results conclusively identify C3G as a multi\u2010target agent against IQ\u2010induced liver damage, highlighting its promise for development into a novel dietary strategy to counteract the toxicological risks of processed meat consumption.<\/jats:p>","DOI":"10.1002\/mnfr.70363","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T15:01:06Z","timestamp":1766761266000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cyanidin\u20103\u2010O\u2010Glucoside Mitigates Hepatotoxicity Induced by 2\u2010Amino\u20103\u2010Methylimidazo[4,5\u2010f]Quinoline via Endogenous and Exogenous Apoptotic Signaling Pathways: Evidence From In Vivo and In Silico Studies"],"prefix":"10.1002","volume":"70","author":[{"given":"Shaoxuan","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Geriatric Nutrition and Health Beijing Technology and Business University Ministry of 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