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Lactic acid bacteria (LAB), particularly\n                      <jats:italic>Levilactobacillus brevis<\/jats:italic>\n                      , have garnered increasing interest for their antimicrobial capabilities in enhancing food safety. This study investigates the antimicrobial activity and secondary metabolite production of cell\u2010free supernatant extract in ethyl acetate of\n                      <jats:italic>L. brevis<\/jats:italic>\n                      MTCC 1750, emphasizing its potential application in food bio\u2010preservation. The strain demonstrated key probiotic attributes, including acid tolerance, strong cell surface hydrophobicity, and 48% auto\u2010aggregation after 12\u00a0h, indicating good survivability and colonization potential.\n                      <jats:italic>L. brevis<\/jats:italic>\n                      MTCC 1750 exhibited broad\u2010spectrum antibacterial and antifungal activity, forming clear inhibition zones. Additionally, it significantly inhibited biofilm formation and quorum sensing (QS)\u2013regulated pigment production, reducing violacein, prodigiosin, and pyocyanin by 52.05%, 90.43%, and 43.73%, respectively. Importantly, metabolomics\u2010guided analyses (FTIR, HPTLC, and HR\u2010LCMS) provided a detailed chemical fingerprint of diverse bioactive metabolites, including peptides, fatty acids, and phenolic compounds, which has not been previously much reported for this strain. Furthermore, the study uniquely demonstrates the dual functionality of this strain, combining probiotic attributes with strong anti\u2010QS and antibiofilm activities, thereby highlighting its novelty compared to earlier investigations. The nonhemolytic activity of the strain further supports its safety. These findings highlight\u00a0\n                      <jats:italic>Levilactobacillus brevis<\/jats:italic>\n                      \u00a0MTCC 1750 as a promising natural biopreservative with potent antimicrobial and antibiofilm properties. The integration of metabolomics\u2010guided secondary metabolite profiling with functional assays represents a novel contribution, positioning this strain as a safe, multifunctional candidate for food safety enhancement and shelf\u2010life extension.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mnfr.70366","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T04:41:56Z","timestamp":1770007316000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Metabolomics\u2010Based Functional Characterization of\n                    <i>Levilactobacillus Brevis<\/i>\n                    : Insights Into Probiotic and Antimicrobial Potential"],"prefix":"10.1002","volume":"70","author":[{"given":"Nosyba A.","family":"Ibrahim","sequence":"first","affiliation":[{"name":"Department of Public Health College of Public Health &amp; Health Informatics University of Ha'il  Ha'il Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9283-2124","authenticated-orcid":false,"given":"Mitesh","family":"Patel","sequence":"additional","affiliation":[{"name":"Faculty of Engineering and Technology Department of Computer Science and Bioscience Marwadi University  Rajkot Gujarat India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhvani","family":"Trivedi","sequence":"additional","affiliation":[{"name":"Research and Development Cell (RDC) Parul University  Vadodara Gujarat India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Komal","family":"Janiyani","sequence":"additional","affiliation":[{"name":"Research and Development Cell (RDC) Parul University  Vadodara Gujarat India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9202-6624","authenticated-orcid":false,"given":"Abdel Moneim Elhadi","family":"Sulieman","sequence":"additional","affiliation":[{"name":"Department of Biology College of Science University of Ha'il  Ha'il Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haroon Elrasheid","family":"Tahir","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering Jiangsu University  Zhenjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6825-1338","authenticated-orcid":false,"given":"Reyaz Hassan","family":"Mir","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Division Department of Pharmaceutical Sciences University of Kashmir, Hazratbal  Srinagar Jammu and Kashmir India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syeda Bushra","family":"Fatima","sequence":"additional","affiliation":[{"name":"Department of Clinical Nutrition College of Applied Medical Science University of Ha'il  Ha'il Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7080-6822","authenticated-orcid":false,"given":"Mohd","family":"Adnan","sequence":"additional","affiliation":[{"name":"Department of Biology College of Science University of Ha'il  Ha'il Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,1]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods5020021"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3934\/microbiol.2017.3.529"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bj.2018.03.003"},{"key":"e_1_2_12_5_1","first-page":"171","volume-title":"Elementary Food Science","author":"Owusu\u2010Apenten R.","year":"2022"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.22271\/chemi.2020.v8.i2f.8794"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2020.109414"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1258\/0007142001902996"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2016.06.020"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.924398"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods11091283"},{"key":"e_1_2_12_12_1","first-page":"1","article-title":"Evaluation of Organic Acids, Anti\u2010Salmonella Activities of Lactic Acid Bacteria Isolated From Nigerian Grown Salad Vegetables","volume":"11","author":"Akanji B. 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