{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T01:05:25Z","timestamp":1783127125954,"version":"3.54.6"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T00:00:00Z","timestamp":1770508800000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Gluten protein generates an immunogenic 33\u2010mer peptide upon incomplete digestion, leading to induced cytotoxic and inflammatory responses in intestinal epithelial cells of susceptible individuals with Celiac disease (CeD). The present study investigates the protective effects of gluten hydrolyzing probiotic strains\u2014\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    AJG10 and\n                    <jats:italic>Bacillus tequilensis<\/jats:italic>\n                    AJG23 on gliadin and 33\u2010mer peptide in the Caco\u20102 cell model. The effect of probiotics on further degradation of 33\u2010mer peptide and pepsin\u2010trypsin digested gliadin was analysed using SDS\u2010PAGE and mass spectrometry. The effect of all fractions on Caco\u20102 cell viability, nitric oxide (NO) production, and expression of pro\u2010inflammatory cytokines was evaluated. A significant degradation of immunogenic peptide fragments after probiotic treatments resulted in 44%\u201353% reduction in NO production and a decrease of 27%\u201337% in cytokine expression and overall improvement in cell viability. The results suggested the potential of probiotic interventions in reducing the toxic effects of digested gliadin and immunogenic peptides.\n                  <\/jats:p>","DOI":"10.1002\/mnfr.70367","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T04:48:48Z","timestamp":1770612528000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Protective Effects of Probiotic Mediated Digested Gliadin and 33\u2010mer Peptide in Caco\u20102 Cell Cultures"],"prefix":"10.1002","volume":"70","author":[{"given":"Anjali","family":"Jaglan","sequence":"first","affiliation":[{"name":"Department of Microbiology Central University of Haryana  Mahendergarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunjan","family":"Sadera","sequence":"additional","affiliation":[{"name":"Department of Microbiology Central University of Haryana  Mahendergarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anju","family":"Nagpal","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Division ICAR\u2010National Dairy Research Institute  Karnal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Payal S.","family":"Mate","sequence":"additional","affiliation":[{"name":"Animal Biochemistry Division ICAR\u2010National Dairy Research Institute  Karnal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudarshan","family":"Kumar","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Division ICAR\u2010National Dairy Research Institute  Karnal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunjan","family":"Goel","sequence":"additional","affiliation":[{"name":"Department of Microbiology Central University of Haryana  Mahendergarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.7326\/AITC202001070"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods14030377"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2024.02.044"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/17474124.2025.2483406"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2024.104113"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","unstructured":"H. M.Ali Z. A.Mohammed andO. K.Abbas \u201cGut Microbiota Dysbiosis in Celiac Disease Exploring the Probiotic Solution \u201dIraqi Journal of Community Medicine38(2025): 96\u201310 https:\/\/doi.org\/10.4103\/IRJCM.IRJCM_45_24.","DOI":"10.4103\/IRJCM.IRJCM_45_24"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-024-10416-y"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/07853890.2021.1990392"},{"key":"e_1_2_8_10_1","volume-title":"StatPearls","author":"Daley S. F.","year":"2025"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10121824"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20103\u2010031\u201058572\u20102_6"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.578386"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10238\u2010022\u201000987\u2010x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu13030992"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fochx.2024.101465"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods13152444"},{"key":"e_1_2_8_18_1","first-page":"1","article-title":"Genomic and Phenotypic Evaluation of the Gliadin\u2010Degrading Probiotic Bacillus Amyloliquefaciens EG025 From Cheonggukjang for Celiac Disease Treatment","volume":"17","author":"Jo J.","year":"2025","journal-title":"Probiotics and Antimicrobial Proteins"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2023.113516"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foohum.2025.100567"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac504689z"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1117\/12.2058730"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.0c01421"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866\u2010017\u20101051\u20101"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2021.110720"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2013.09.055"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12020495"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602\u2010020\u201009723\u2010x"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365520500235334"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2025.106759"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00260-07"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22459"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300\u2010483X(99)00223\u20101"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014\u20105793(03)00236\u20109"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf201212m"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.70222"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2012.06.007"},{"key":"e_1_2_8_40_1","doi-asserted-by":"crossref","unstructured":"O.Nikoloudaki G.Celano A.Polo et\u00a0al. \u201cNovel Probiotic Preparation With In Vivo Gluten\u2010Degrading Activity and Potential Modulatory Effects on the Gut Microbiota \u201dMicrobiology Spectrum12(2024): e03524\u2013e03523 https:\/\/doi.org\/10.1128\/spectrum.03524\u201023.","DOI":"10.1128\/spectrum.03524-23"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.26599\/FSHW.2024.9250033"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11694\u2010023\u201002267\u2010y"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2005.117"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2018.06.020"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2000.278.6.G886"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.70367","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mnfr.70367","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.70367","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T09:16:43Z","timestamp":1772961403000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.70367"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":44,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/mnfr.70367"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.70367","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"value":"1613-4125","type":"print"},{"value":"1613-4133","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"2025-05-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70367"}}