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Plasma hormones (ELISA), inflammatory biomarkers (electrochemiluminescence), splenocytes phenotype (immunofluorescence), and\n                    <jats:italic>ex vivo<\/jats:italic>\n                    cytokine production (ELISA) after mitogen stimulation were measured. HFD did not alter body weight\/composition or inflammatory profile but increased plasma glucagon\u2010like peptide 1 (GLP\u20101, males,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.02). In the spleen, HFD lowered transforming growth factor\u2010beta (TGF\u2010\u03b2) and increased interleukin (IL)\u201010 (females) and IL\u20102 production (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.03). It also lowered % of regulatory T cells (Treg), B cells (males), conventional type 1dendritic cells (cDC1), and MHC\u2010II and CD86 expression (males) (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.03). DHA mitigated HFD\u2010related changes in plasma GLP\u20101 (males) andTGF\u2010\u03b2, IL\u201010 (females), and IL\u20102 production (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0\u2264\u00a00.03) and increased % of B cells (males), cDC1, MHC\u2010II, and CD86 expression (males) (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.03).\n                  <\/jats:p>\n                  <jats:p>DHA mitigated HFD\u2010induced impaired Treg response and compromised adaptive immunity and antigen\u2010presenting function in a sex\u2010specific manner.<\/jats:p>","DOI":"10.1002\/mnfr.70391","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T06:17:20Z","timestamp":1769062640000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Docosahexaenoic Acid Consumption Early in Life Improves Immune Dysfunction in 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