{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T14:26:29Z","timestamp":1780496789704,"version":"3.54.1"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The growing demand for sustainable and functional food ingredients has driven interest in marine microalgae as a source of bioactive compounds. This study evaluates the bioactivity, safety, and metabolite profile of extracts from\n                    <jats:italic>Dunaliella salina<\/jats:italic>\n                    and\n                    <jats:italic>Pavlova gyrans<\/jats:italic>\n                    , produced through two extraction methods: osmotic shock and bead milling with ethanol. Aqueous extracts were rich in peptides and amino acids, while ethanolic extracts contained higher pigment concentrations, underlining distinct nutritional biochemistry profiles. Both extracts exhibited significant antioxidant activity (4\u00a0mg mL\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    ), with aqueous extracts demonstrating a stronger effect. Cellular antioxidant activity assays using the Caco\u20102 cell line confirmed comparable efficacy between extraction methods. Antibacterial tests revealed that\n                    <jats:italic>D. salina<\/jats:italic>\n                    extracts effectively inhibited\n                    <jats:italic>Listeria innocua<\/jats:italic>\n                    ,\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    , and\n                    <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                    , while\n                    <jats:italic>P. gyrans<\/jats:italic>\n                    extracts showed activity against\n                    <jats:italic>L. innocua<\/jats:italic>\n                    ,\n                    <jats:italic>E. coli<\/jats:italic>\n                    ,\n                    <jats:italic>Bacillus cereus<\/jats:italic>\n                    , and\n                    <jats:italic>Pseudomonas fluorescens<\/jats:italic>\n                    . Additionally, both microalgae extracts demonstrated functional food potential by inhibiting \u03b1\u2010glucosidase (27%\u201336%) and angiotensin\u2010converting enzyme (21.7%\u201337.9%), suggesting antidiabetic and antihypertensive properties. Cell viability assays confirmed the extracts\u2019 safety for potential food applications. These findings support the sustainable use of\n                    <jats:italic>D. salina<\/jats:italic>\n                    and\n                    <jats:italic>P. gyrans<\/jats:italic>\n                    as innovative bioactive ingredients for functional and eco\u2010friendly food formulations.\n                  <\/jats:p>","DOI":"10.1002\/mnfr.70398","type":"journal-article","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T04:09:43Z","timestamp":1769659783000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Assessment of Marine Microalgae's Bioactive Extracts Potential for Food Applications"],"prefix":"10.1002","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3117-4543","authenticated-orcid":false,"given":"V.","family":"Sousa","sequence":"first","affiliation":[{"name":"Centre of Biological Engineering (CEB) University of Minho  Braga Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Coelho","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro De Biotecnologia e Qu\u00edmica Fina\u2014Laborat\u00f3rio Associado Escola Superior de Biotecnologia Universidade Cat\u00f3lica Portuguesa  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Martins","sequence":"additional","affiliation":[{"name":"Centre of Biological Engineering (CEB) University of Minho  Braga Portugal"},{"name":"LABBELS Associate Laboratory  Braga\/Guimar\u00e3es Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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