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Plant\u2010derived extracellular vesicles have recently emerged as functional nanocarriers of bioactive metabolites; however, these vesicles from\n                    <jats:italic>P. peruviana<\/jats:italic>\n                    have not been systematically investigated. We isolated and characterized exosomes from\n                    <jats:italic>P. peruviana<\/jats:italic>\n                    fruits and evaluated their anti\u2010inflammatory and antioxidant activities in vitro. Vesicles were isolated and characterized using transmission electron microscopy, nanoparticle tracking analysis, protein and RNA profiling, thin\u2010layer chromatography, and untargeted metabolomics. The vesicles exhibited characteristic exosomal morphology with a mean diameter of \u223c142\u00a0nm and contained proteins, miRNAs, lipids, and \u03b2\u2010carotene as a predominant metabolite. Functional assays revealed that, at non\u2010cytotoxic concentrations (25\u00a0\u00b5g\/mL), they significantly suppressed IL\u20106, TNF\u2010\u03b1, IFN\u2010\u03b2, iNOS, and STAT1 expression in LPS\u2010stimulated RAW 264.7 macrophages, accompanied by reduced IKK phosphorylation and inhibition of NF\u2010\u03baB and STAT1 transcriptional activity. In H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010challenged NIH3T3 fibroblasts, exosome treatment enhanced cell viability and upregulated endogenous antioxidant and mitochondrial defense markers, including CAT, SOD2, GPx4, and cytochrome c oxidase. These findings identify P. peruviana\u2013derived exosomes as carotenoid\u2010rich nanocarriers with anti\u2010inflammatory and antioxidant properties, providing new mechanistic insight into the molecular actions of carotenoid\u2010containing foods.\n                  <\/jats:p>","DOI":"10.1002\/mnfr.70458","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T07:50:11Z","timestamp":1775548211000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Therapeutic Modulation of Inflammatory Signaling via\n                    <i>Physalis Peruviana<\/i>\n                    \u2010Derived Exosomes: A Targeted Effect on IKK\/NF\u03baB\/STAT1"],"prefix":"10.1002","volume":"70","author":[{"given":"Cristian","family":"Mart\u00ednez Fajardo","sequence":"first","affiliation":[{"name":"Instituto Bot\u00e1nico. Departamento de Ciencia y Tecnolog\u00eda Agroforestal y Gen\u00e9tica. 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