{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T12:11:20Z","timestamp":1783944680409,"version":"3.55.0"},"reference-count":14,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Coffee extracts contain numerous bioactive compounds. Given the dietary link between coffee consumption and colorectal cancer, this study compared the effects of roasted and green (unroasted) coffee extracts on human colorectal cancer cells (HCT116) and non\u2010cancerous fibroblasts (BJ\u20105ta) to evaluate how processing influences proliferation and molecular signaling. Real\u2010time cell analysis (RTCA), qRT\u2010PCR, and label\u2010free quantitative proteomic analysis were performed to assess cellular responses. MTS and RTCA showed that in BJ\u20105Ta fibroblasts, coffee extracts increased proliferation in the order CNR &lt; CAR &lt; CAU &lt; CNU, whereas the trend was reversed in HCT116 cancer cells. Proteomic analysis revealed that in BJ\u20105Ta cells, unroasted coffee extract caused downregulation of the ribosome pathway, and natural coffee extract caused downregulation of the gap junction pathway, indicating reduced protein synthesis and cell\u2013cell communication as a potential stress\u2010adaptive response. In contrast, in HCT116 cells, unroasted coffee extract upregulated the ribosome pathway. Also, natural coffee extract upregulated the pentose phosphate pathway in HCT116 cells, which may enhance NADPH production and reduce oxidative stress. Current evidence suggests coffee's bioactive compounds may have different effects varying by coffee extract type and their preparation.<\/jats:p>","DOI":"10.1002\/mnfr.70486","type":"journal-article","created":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T05:23:51Z","timestamp":1777699431000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Assessment the Effects of Roasted and Non\u2010Roasted Coffee Extracts on Colon Cancer Cells"],"prefix":"10.1002","volume":"70","author":[{"given":"M.","family":"\u0160emel\u00e1kov\u00e1","sequence":"first","affiliation":[{"name":"Department of Medical Biology Faculty of Medicine Pavol Jozef \u0160af\u00e1rik University  Ko\u0161ice Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Bober","sequence":"additional","affiliation":[{"name":"Department of Medical and Clinical Biophysics Faculty of Medicine Pavol Jozef \u0160af\u00e1rik University  Ko\u0161ice Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"V\u00e1rady","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene Technology and Safety University of Veterinary Medicine and Pharmacology  Ko\u0161ice Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Hud\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Medical Biology Faculty of Medicine Pavol Jozef \u0160af\u00e1rik University  Ko\u0161ice Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Harvanik","sequence":"additional","affiliation":[{"name":"Department of Medical Biology Faculty of Medicine Pavol Jozef \u0160af\u00e1rik University  Ko\u0161ice Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"\u0160alagovi\u010d","sequence":"additional","affiliation":[{"name":"Department of Medical Biology Faculty of Medicine Pavol Jozef \u0160af\u00e1rik University  Ko\u0161ice Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Popelka","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene Technology and Safety University of Veterinary Medicine and Pharmacology  Ko\u0161ice Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Sol\u00e1r","sequence":"additional","affiliation":[{"name":"Department of Medical Biology Faculty of Medicine Pavol Jozef \u0160af\u00e1rik University  Ko\u0161ice Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,1]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.14735\/amko2017106"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12263-015-0501-3"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14224880"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5572630"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24032706"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.afres.2024.100512"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/app12041938"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2020.110014"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph14080761"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0RA01179G"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29010088"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2017.6227"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11122458"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2019.110595"}],"container-title":["Molecular Nutrition &amp; 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