{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T12:12:57Z","timestamp":1783944777657,"version":"3.55.0"},"reference-count":64,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Helicobacter pylori<\/jats:italic>\n                    infection remains a major global health concern due to its association with gastric inflammation, ulceration, and gastric malignancies. This study evaluated the effects of Ngoc Linh ginseng (\n                    <jats:italic>Panax vietnamensis<\/jats:italic>\n                    Ha et Grushv.) root fractions on\n                    <jats:italic>H. pylori<\/jats:italic>\n                    virulence and host inflammatory responses. UHPLC\u2010MS\/MS\u2010based metabolomic profiling coupled with feature\u2010based molecular networking was employed to characterize the chemical profiles of different solvent fractions, identifying the dichloromethane (DCM) fraction as enriched in ginsenosides, particularly the ocotillol\u2010type saponin majonoside R2 (MR2). In vitro assays showed that, despite minimal direct antibacterial activity, the DCM fraction at sub\u2010inhibitory concentrations significantly reduced urease activity, acid tolerance, biofilm formation, and the expression of major virulence genes, including\n                    <jats:italic>vacA<\/jats:italic>\n                    and\n                    <jats:italic>cagA<\/jats:italic>\n                    . In\n                    <jats:italic>H. pylori<\/jats:italic>\n                    \u2010infected AGS gastric epithelial cells, the DCM fraction and MR2 decreased VacA and CagA translocation, suppressed pro\u2010inflammatory signaling and cytokine production, restored antioxidant defenses, and alleviated mitochondrial apoptosis. By contrast, ginsenoside Rg1 selectively modulated host inflammatory and oxidative stress responses without affecting bacterial virulence gene expression. These results demonstrate that Ngoc Linh ginseng root fractions mitigate\n                    <jats:italic>H. pylori<\/jats:italic>\n                    \u2010induced pathogenic effects primarily through anti\u2010virulence and host\u2010directed mechanisms, highlighting their potential relevance for the development of gastric health\u2010promoting functional products.\n                  <\/jats:p>","DOI":"10.1002\/mnfr.70493","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T09:26:14Z","timestamp":1778491574000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Alleviation of\n                    <i>Helicobacter pylori<\/i>\n                    \u2010Induced Pathogenicity and Gastric Inflammation by Majonoside\u2010R2\u2010 and Ginsenoside Rg1\u2010Rich Fractions From\n                    <i>Panax vietnamensis<\/i>\n                    Ha Et Grushv.: A Metabolomics\u2010Guided Investigation"],"prefix":"10.1002","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3198-3383","authenticated-orcid":false,"given":"Anh Duy","family":"Do","sequence":"first","affiliation":[{"name":"CIRTech Institute HUTECH University  Ho Chi Minh City Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6816-2407","authenticated-orcid":false,"given":"Thanh\u2010Sang","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Food Technology Saigon Technology University Ho Chi Minh City Vietnam"},{"name":"PhD Program in Clinical Drug Development of Herbal Medicine College of Pharmacy Taipei Medical University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1471-6352","authenticated-orcid":false,"given":"Thi\u2010Van\u2010Linh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Applied Science and Technology (FAST) Nguyen Tat Thanh University  Ho Chi Minh City Vietnam"},{"name":"VINACROP Company Limited  Ho Chi Minh City Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3071-2079","authenticated-orcid":false,"given":"Thi Tuong Vi","family":"Tran","sequence":"additional","affiliation":[{"name":"Faculty of Applied Science and Technology (FAST) Nguyen Tat Thanh University  Ho Chi Minh City Vietnam"},{"name":"VINACROP Company Limited  Ho Chi Minh City Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3653-7881","authenticated-orcid":false,"given":"Tuan\u2010Kiet","family":"Ly","sequence":"additional","affiliation":[{"name":"Center of Analytical Services, Experimentation and Standards, Metrology, Quality, HCM city (CASE\u2010SMQ) Ho Chi Minh City Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nam\u2010Phuong","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Center of Analytical Services, Experimentation and Standards, Metrology, Quality, HCM city (CASE\u2010SMQ) Ho Chi Minh City Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4243-4547","authenticated-orcid":false,"given":"Chung\u2010Kuang","family":"Lu","sequence":"additional","affiliation":[{"name":"PhD Program in Clinical Drug Development of Herbal Medicine College of Pharmacy Taipei Medical University  Taipei Taiwan"},{"name":"National Research Institute of Chinese Medicine  Taipei Taiwan"},{"name":"Department of Life Sciences and Institute of Genome Sciences National Yang Ming Chiao Tung University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9097-4471","authenticated-orcid":false,"given":"Quoc\u2010Duy","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Applied Science and Technology (FAST) Nguyen Tat Thanh University  Ho Chi Minh City Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/1040841X.2021.1975643"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/16833_2022_31"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.13084"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants13223117"},{"key":"e_1_2_10_6_1","article-title":"Recent Advances in Anti\u2010Virulence Therapeutic Strategies With a Focus on Dismantling Bacterial Membrane Microdomains, Toxin Neutralization, Quorum\u2010Sensing Interference and Biofilm Inhibition","volume":"9","author":"Mart\u00ednez O. 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