{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T14:06:18Z","timestamp":1784037978298,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004772","name":"Natural Science Foundation of Ningxia Province","doi-asserted-by":"publisher","award":["2023AAC03549"],"award-info":[{"award-number":["2023AAC03549"]}],"id":[{"id":"10.13039\/501100004772","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study aimed to elucidate the role of the TLR4 pathway in the antiepileptic effects of sulforaphane (SFN). C57BL\/6 mice were randomized into control, SFN, epilepsy (EP), and SFN intervention groups. SFN was administered for 7 days (25\u00a0mg\/kg\/day, oral gavage) to the intervention and SFN groups before epilepsy was induced. Epilepsy was induced in the EP and intervention groups using a lithium chloride\u2013pilocarpine protocol. Behavioral and electroencephalogram (EEG) data were collected. Hippocampal tissue was examined for inflammatory markers and histopathological changes, and protein expression in the TLR4\/NF\u2011\u03baB\/NLRP3 pathway was assessed by western blot. The involvement of TLR4 was further validated through intraperitoneal administration of the selective inhibitor TAK\u2011242 (3.0\u00a0mg\/kg). SFN pretreatment significantly ameliorated seizure severity, mortality, pathologically elevated EEG delta wave frequency, hippocampal pro\u2011inflammatory cytokine levels, and neuronal damage (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05). Molecular analysis showed that SFN reduced the expression of TLR4, MyD88, p\u2010NF\u2010\u03baB, NLRP3, and caspase\u20101 (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05). SFN combined with TAK\u2010242 did not further enhance the reduction of these indicators, compared with SFN alone. The TLR4\/NF\u2010\u03baB\/NLRP3 signaling pathway plays a crucial role in SFN\u2010mediated attenuation of neuroinflammation and contributes to its antiepileptic and neuroprotective effects.\n                  <\/jats:p>","DOI":"10.1002\/mnfr.70510","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T08:31:13Z","timestamp":1780302673000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeting TLR4\u2010Depended NLRP3 Inflammasome: Sulforaphane's Mechanism in Suppressing Neuroinflammation and Seizures in Epileptic Mice"],"prefix":"10.1002","volume":"70","author":[{"given":"Guoli","family":"Zhou","sequence":"first","affiliation":[{"name":"Ningxia Key Laboratory of Craniocerebral Diseases Ningxia Medical University  Yinchuan China"},{"name":"School of Public Health Ningxia Medical University Ningxia Medical University  Yinchuan China"},{"name":"Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Jin","sequence":"additional","affiliation":[{"name":"Medical Records Department General Hospital of Ningxia Medical University  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingwen","family":"Wang","sequence":"additional","affiliation":[{"name":"Ningxia Key Laboratory of Craniocerebral Diseases Ningxia Medical University  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiandong","family":"Jia","sequence":"additional","affiliation":[{"name":"Ningxia Key Laboratory of Craniocerebral Diseases Ningxia Medical University  Yinchuan China"},{"name":"School of Public Health Ningxia Medical University Ningxia Medical University  Yinchuan China"},{"name":"Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control  Yinchuan 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