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This study integrated 16S rRNA sequencing, untargeted metabolomics, and molecular analyses to evaluate their therapeutic potential in an AFLD mouse model. Results indicated that RJ (200\u00a0mg\/kg\/day) and 10\u2010HDA (100\u00a0mg\/kg\/day) alleviated alcohol\u2010induced liver injury by reducing lipid accumulation, dyslipidemia, inflammation, and oxidative stress. Furthermore, these interventions modulated gut microbiota composition by decreasing the relative abundance of\n                    <jats:italic>Pseudomonadota<\/jats:italic>\n                    and\n                    <jats:italic>Escherichia<\/jats:italic>\n                    , while increasing taxa such as\n                    <jats:italic>Akkermansia<\/jats:italic>\n                    and\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    . Metabolomic profiling suggested the involvement of key pathways, including serotonergic synapse, bile secretion, and tryptophan metabolism. In the liver, RJ and 10\u2010HDA treatment was associated with the activation of the AMPK pathway, which promotes fatty acid \u03b2\u2010oxidation and suppresses lipogenesis. Notably, integrated correlation analyses indicated that the restoration of certain fecal metabolites correlated with hepatic AMPK activation. Collectively, RJ and 10\u2010HDA may mitigate AFLD by modulating the gut\u2010microbiota\u2010metabolite axis and the AMPK signaling pathway, supporting their potential use as dietary supplements for liver health.\n                  <\/jats:p>","DOI":"10.1002\/mnfr.70511","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T08:38:52Z","timestamp":1780303132000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Royal Jelly and 10\u2010Hydroxy\u20102\u2010decenoic Acid Mitigate Alcoholic Fatty Liver Disease in Mice via the Gut\u2010Microbiota\u2010Metabolite Axis"],"prefix":"10.1002","volume":"70","author":[{"given":"Lining","family":"Ren","sequence":"first","affiliation":[{"name":"College of Biological Science and Technology Beijing Key Laboratory of Forest Food Processing and Safety Beijing Forestry University  Beijing China"},{"name":"Hebei Province Key Laboratory of Sustainable 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhang","family":"Xiao","sequence":"additional","affiliation":[{"name":"College of Biological Science and Technology Beijing Key Laboratory of Forest Food Processing and Safety Beijing Forestry University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijing","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Biological Science and Technology Beijing Key Laboratory of Forest Food Processing and Safety Beijing Forestry University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guochen","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Biological Science and Technology Beijing Key Laboratory of Forest Food Processing and Safety Beijing Forestry University  Beijing 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