{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T14:07:05Z","timestamp":1784038025545,"version":"3.55.0"},"reference-count":44,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This exploratory study investigated the interactive effects of dietary fat content and methyl\u2010donor supplementation on genome\u2010wide DNA methylation in adipose tissue of female lupus\u2010prone NZBWF1\/J mice. Thirty mice were randomly assigned to four groups for 12 weeks in a 2\u00a0\u00d7\u00a02 factorial design: standard diet (SD,\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a07), high\u2010fat diet (HFD,\n                    <jats:italic>n<\/jats:italic>\n                    = 7), standard diet supplemented (SDS,\n                    <jats:italic>n<\/jats:italic>\n                    = 8), and high\u2010fat diet supplemented (HFDS,\n                    <jats:italic>n<\/jats:italic>\n                    = 8). The standard diet provided 4.2\u00a0kcal\/g and the high\u2010fat diet 6.6\u00a0kcal\/g. Supplemented diets contained 8\u00a0mg\/kg folic acid and 50\u00a0\u00b5g\/kg vitamin B12. After a 10\u2010h fast, subcutaneous adipose tissue was collected for genome\u2010wide DNA methylation analysis using the Infinium Mouse Methylation BeadChip, followed by differential methylation and pathway enrichment analyses. The high\u2010fat diet induced a distinct methylation profile with 90 differentially methylated CpGs (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05, \u0394\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u00a0\u2265\u00a00.05). Enrichment analyses did not identify significantly overrepresented pathways. Diet \u00d7 supplementation interaction analysis identified 31 CpGs with significant interaction effects (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.01). Pathway enrichment analysis revealed overrepresentation of the IL\u201017 signaling pathway. Only one CpG overlapped between HFD\u2010associated and interaction\u2010associated loci, indicating largely distinct epigenetic responses to diet and supplementation. A high\u2010fat diet induces widespread epigenetic remodeling in adipose tissue, while folic acid and vitamin B12 supplementation modulates inflammatory epigenetic pathways in a diet\u2010dependent manner.\n                  <\/jats:p>","DOI":"10.1002\/mnfr.70512","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T06:08:32Z","timestamp":1781244512000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome\u2010Wide DNA Methylation Changes Induced by High\u2010Fat Diet and Methyl Donor Supplementation in Female Lupus Models: An Exploratory Study"],"prefix":"10.1002","volume":"70","author":[{"given":"Amanda Alves","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Applied Physiology and Nutrition Research Group \u2013 School of Physical Education and Sport and Faculdade de Medicina FMUSP Universidade De Sao Paulo  Brazil"},{"name":"Center of Lifestyle Medicine, Faculdade de Medicina FMUSP Universidade De Sao Paulo  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Moura","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Applied Physiology and Nutrition Research Group \u2013 School of Physical Education and Sport and Faculdade de Medicina FMUSP Universidade De Sao Paulo  Brazil"},{"name":"Center of Lifestyle Medicine, Faculdade de Medicina FMUSP Universidade De Sao Paulo  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jhulia Caroline Nunes Leal","family":"da Mota","sequence":"additional","affiliation":[{"name":"Applied Physiology and Nutrition Research Group \u2013 School of Physical Education and Sport and Faculdade de Medicina FMUSP Universidade De Sao Paulo  Brazil"},{"name":"Center of Lifestyle Medicine, Faculdade de Medicina FMUSP Universidade De Sao Paulo  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia Lobato","family":"Souza","sequence":"additional","affiliation":[{"name":"Applied Physiology and Nutrition Research Group \u2013 School of Physical Education and Sport and Faculdade de Medicina FMUSP Universidade De Sao Paulo  Brazil"},{"name":"Center of Lifestyle Medicine, Faculdade de Medicina FMUSP Universidade De Sao Paulo  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela Augusta de Souza","family":"Pinhel","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo  Brazil"},{"name":"Division of Nutrition and Metabolism, Department of Health Sciences, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla Barbosa","family":"Nonino","sequence":"additional","affiliation":[{"name":"Division of Nutrition and Metabolism, Department of Health Sciences, Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bidossessi Wilfried","family":"Hounkpe","sequence":"additional","affiliation":[{"name":"Rheumatology Division, Hospital das Clinicas HCFMUSP, Faculdade de Medicina Universidade De S\u00e3o Paulo  Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walcy Paganelli Rosolia","family":"Teodoro","sequence":"additional","affiliation":[{"name":"Rheumatology Division, Hospital das Clinicas HCFMUSP, Faculdade de Medicina Universidade De S\u00e3o Paulo  Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Costa\u2010Fraga","sequence":"additional","affiliation":[{"name":"Epigenomics Unit, Cancer Epigenomics, Translational Medical Oncology Group (ONCOMET); Clinical University Hospital &amp; Health Research Institute of Santiago de Compostela. CIBERONC University of Santiago de Compostela, Santiago de Compostela  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea Gonzales","family":"Izquierdo","sequence":"additional","affiliation":[{"name":"Epigenomics in Endocrinology and Nutrition Group, Epigenomics Unit, Health Research Institute of Santiago de Compostela (IDIS) University Clinical Hospital of Santiago (CHUS, SERGAS), Santiago de Compostela  Spain"},{"name":"Centro De Investigaci\u00f3n Biom\u00e9dica En Red Fisiopatolog\u00eda De La Obesidad y Nutrici\u00f3n (CIBERobn) Instituto De Salud Carlos III (ISCIII)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"Diaz\u2010Lagares","sequence":"additional","affiliation":[{"name":"Epigenomics Unit, Cancer Epigenomics, Translational Medical Oncology Group (ONCOMET); Clinical University Hospital &amp; Health Research Institute of Santiago de Compostela. 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