{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T07:04:54Z","timestamp":1785308694067,"version":"3.55.0"},"reference-count":44,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Obesity induced by high\u2011fat, high\u2011sucrose diets (HFSD) remains a major global health challenge, disrupting lipid metabolism, glucose homeostasis, and gut microbial balance. These disturbances underscore the need for safe interventions capable of restoring metabolic regulation.\n                    <jats:italic>Senna alexandrina<\/jats:italic>\n                    (SA), traditionally used for weight reduction, has recently been shown to modulate the gut microbiota beyond its laxative effects. To enhance efficacy while minimizing adverse outcomes, this study investigated the effects of a low and safe dose of SA combined with intermittent fasting (IF), a strategy that reshapes nutrient availability and microbial dynamics. HFSD\u2011induced obese rats were treated for four weeks with SA leaf powder (300\u00a0mg\/kg\/day), IF, or both. Bioactive constituents of SA were characterized using LC\u2011HRMS, alongside systematic evaluation of physiological and metabolic parameters\u2014including adiposity, morphometry, lipid profiles, insulin sensitivity markers, and gut\u2010endocrine axis indicators. SA supplementation showed improvements across all parameters, surpassing those achieved with IF alone. Importantly, the combined intervention (SA+IF) yielded complementary benefits, notably enhancing GLUT4 expression, short\u2011chain fatty acid (SCFA) production, peptide YY (PYY) secretion, and reducing the Firmicutes\/Bacteroidetes ratio. These findings highlight the complementary roles of SA and IF in metabolic regulation, providing experimental evidence for a potential dietary approach to mitigate obesity\u2011related dysfunction.\n                  <\/jats:p>","DOI":"10.1002\/mnfr.70531","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T08:05:21Z","timestamp":1785139521000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Intermittent Fasting Amplifies Gut\u2013Endocrine Axis Responses to\n                    <i>Senna alexandrina<\/i>\n                    Supplementation in Obese Rats"],"prefix":"10.1002","volume":"70","author":[{"given":"Faizah","family":"Fulyani","sequence":"first","affiliation":[{"name":"Department of Medical Biology and Biochemistry, Faculty of Medicine Diponegoro University  Semarang Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iftitan Setya","family":"Widayanti","sequence":"additional","affiliation":[{"name":"Department of Nutrition Science, Faculty of Medicine Diponegoro University  Semarang Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salwa Alifa","family":"Bestari","sequence":"additional","affiliation":[{"name":"Nutrition Science Study Program, Faculty of Health Sciences Universitas Muhammadiyah Surakarta  Surakarta Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lusiana","family":"Batubara","sequence":"additional","affiliation":[{"name":"Department of Medical Biology and Biochemistry, Faculty of Medicine Diponegoro University  Semarang Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.R. Mahayu Dewi","family":"Ariani","sequence":"additional","affiliation":[{"name":"Department of Medical Biology and Biochemistry, Faculty of Medicine Diponegoro University  Semarang Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlie","family":"Roosevelt D.H.","sequence":"additional","affiliation":[{"name":"Medical Study Program, Faculty of Medicine Diponegoro University Semarang Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Ichsanul","family":"Fikri","sequence":"additional","affiliation":[{"name":"Medical Study Program, Faculty of Medicine Diponegoro University Semarang Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galen","family":"Chandrawira","sequence":"additional","affiliation":[{"name":"Medical Study Program, Faculty of Medicine Diponegoro University Semarang Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rio Jati","family":"Kusuma","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Health, Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada  Yogyakarta Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,27]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metop.2025.100375"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/dom.16263"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/obr.13956"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/oby.24239"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.24420"},{"key":"e_1_2_12_7_1","first-page":"234","article-title":"Obesity and Gut\u2010Brain Axis","volume":"19","author":"Micic D. 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Final rule \u201dFederal Register 64 (1999):4535\u20134540."},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo13040559"},{"key":"e_1_2_12_19_1","doi-asserted-by":"crossref","DOI":"10.1177\/1934578X251319221","article-title":"Determination of Total Flavonoid Content and Phenolic Content, Antioxidant Assay, and Antiepileptic Activity of Achillea millefolium Extract","volume":"20","author":"Hayat S.","year":"2025","journal-title":"Natural Products Communications"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27175590"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0250261"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-003-0534-z"},{"key":"e_1_2_12_23_1","first-page":"5","volume-title":"\u201cSenna alexandrina Mill. 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