{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T06:48:50Z","timestamp":1782456530425,"version":"3.54.5"},"reference-count":59,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The intestinal porcine epithelial cell line (IPEC\u2013J2) is a nontransformed jejunal model derived from unsuckled piglets. It is increasingly used in gut physiology research, yet its application to nutrient absorption remains underexplored. Four media types were assessed for their ability to support IPEC\u2013J2 barrier maturation and permeability: Dulbecco's modified Eagle medium\/Ham's F\u201012 with 10% fetal bovine serum (FBS10), 10% porcine serum (PS10), 5% porcine serum (PS5) supplemented with Wnt3a\u2010R\u2010spondin\u2010Noggin conditioned medium (PS5+L\u2010WRN), and PS5 with epidermal growth factor and insulin\u2010transferrin\u2010selenium (PS5+EGF+ITS). Culture medium influenced epithelial phenotype, with more than a 30\u2010fold range in transepithelial electrical resistance (TEER), which plateaued by day 12. PS10 supported a balanced profile, characterized by intermediate TEER (2395.00\u00a0\u00b1\u00a0485.51\u00a0\u03a9\n                    <jats:italic>\u00a0\u00d7\u00a0<\/jats:italic>\n                    cm\n                    <jats:sup>2<\/jats:sup>\n                    ), lowest lactulose and mannitol permeability (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05), high lactate dehydrogenase activity (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05\u00a0vs. PS5+L\u2010WRN and FBS10), enhanced membrane localization of tight junction proteins (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05\u00a0vs. PS5+EGF+ITS), and reduced fibroblast marker S100A4 (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05\u00a0vs. PS5+EGF+ITS). Treatment with digested infant milk formula did not compromise barrier integrity in PS10\u2010cultured IPEC\u2010J2 monolayers and enabled transport of free amino acids to the basolateral compartment. These findings support IPEC\u2010J2 as an alternative to Caco\u20102 cells for applications in infant nutrition research in vitro.\n                  <\/jats:p>","DOI":"10.1002\/mnfr.70540","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T06:02:02Z","timestamp":1782453722000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Culture Optimization of the IPEC\u2013J2 Piglet Jejunum Cell Line for Applications in Infant Nutrition Research"],"prefix":"10.1002","volume":"70","author":[{"given":"Francesca","family":"Bietto","sequence":"first","affiliation":[{"name":"Teagasc Food Research Centre  Fermoy Ireland"},{"name":"School of Food and Nutritional Sciences University College Cork  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myriam M.\u2010L.","family":"Grundy","sequence":"additional","affiliation":[{"name":"INRAE, French National Research Institute for Agriculture, Food and Environment UMR PEGASE, Institut Agro  Saint Gilles France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Arranz","sequence":"additional","affiliation":[{"name":"Secci\u00f3n Departamental de Ciencias de la Alimentaci\u00f3n Universidad Aut\u00f3noma de Madrid (UAM)  Madrid Spain"},{"name":"Institute of Food Science Research CIAL (CSIC\u2010UAM)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Wiart\u2010Letort","sequence":"additional","affiliation":[{"name":"INRAE, French National Research Institute for Agriculture, Food and Environment UMR PEGASE, Institut Agro  Saint Gilles France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Rath","sequence":"additional","affiliation":[{"name":"Chair of Nutrition and Immunology, School of Life Sciences Technische Universit\u00e4t M\u00fcnchen  Freising Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice J.","family":"Lucey","sequence":"additional","affiliation":[{"name":"School of Food and Nutritional Sciences University College Cork  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Giblin","sequence":"additional","affiliation":[{"name":"Teagasc Food Research Centre  Fermoy Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-018-0126-x"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2020.08.004"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2020-322260"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpg.2016.02.007"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00088.2014"},{"key":"e_1_2_11_7_1","first-page":"103","volume-title":"The Impact of Food Bioactives on Health: In Vitro and Ex Vivo Models","author":"Lea T.","year":"2015"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1517\/17425255.4.4.395"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/2211068214561025"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-005-0085-6"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.303"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1536-4801.1995.tb11955.x"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0000000000001610"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.59.3.236"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0954422416000020"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.4161\/gmic.23867"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3945\/an.116.013243"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1536-4801.1995.tb11956.x"},{"key":"e_1_2_11_19_1","first-page":"A41","article-title":"Development of Normal Cultured Small Intestinal Epithelial Cell Lines Which Transport Na and Cl","volume":"96","author":"Berschneider H. 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