{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T05:21:40Z","timestamp":1736227300987,"version":"3.32.0"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["5R01CA212382\u201005","5R01EB024343\u201004","1R03EB032038\u201001"],"award-info":[{"award-number":["5R01CA212382\u201005","5R01EB024343\u201004","1R03EB032038\u201001"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007540","name":"National Metal and Materials Technology Center","doi-asserted-by":"publisher","award":["22\u201002\u2010MPAI\u2010115"],"award-info":[{"award-number":["22\u201002\u2010MPAI\u2010115"]}],"id":[{"id":"10.13039\/501100007540","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aapm.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Physics"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Phase\u2010contrast computed tomography (CT) using high\u2010brilliance, synchrotron\u2010generated x\u2010rays enable three\u2010dimensional (3D) visualization of microanatomical structures within biological specimens, offering exceptionally high\u2010contrast images of soft tissues. Traditional methods for phase\u2010contrast CT; however, necessitate a gap between the subject and the x\u2010ray camera, compromising spatial resolution due to penumbral blurring. Our newly developed technique, Superimposed Wavefront Imaging of Diffraction\u2010enhanced x\u2010rays (SWIDeX), leverages a Laue\u2010case Si angle analyzer affixed to a scintillator to convert x\u2010rays to visible light, capturing second\u2010order differential phase contrast images and effectively eliminating the distance to the x\u2010ray camera. This innovation achieves superior spatial resolution over conventional methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Purpose<\/jats:title><jats:p>In this paper, the imaging principle and CT reconstruction algorithm based on SWIDeX are presented in detail and compared with conventional analyzer\u2010based imaging (ABI). It also shows the physical setup of SWIDeX that provides the resolution preserving second\u2010order differential images for reconstruction. We compare the spatial resolution and the sensitivity of SWIDeX to conventional ABI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To demonstrate high\u2010spatial resolution achievable by SWIDeX, the internal structures of four human tissues\u2014ductal carcinoma in situ, normal stomach, normal pancreas, and intraductal papillary mucinous neoplasm of the pancreas\u2014were visualized using an imaging system configured at the Photon Factory's BL14B beamline under the High Energy Accelerator Research Organization (KEK). Each tissue was thinly sliced after imaging, stained with hematoxylin and eosin (H&amp;E) for conventional microscope\u2010based pathology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A comparison of SWIDeX\u2010CT and pathological images visually demonstrates the effectiveness of SWIDeX\u2010CT for biological tissue imaging. SWIDeX could generate clearer 3D images than existing analyzer\u2010based phase\u2010contrast methods and accurately delineate tissue structures, as validated against histopathological images.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>SWIDeX can visualize important 3D structures in biological soft tissue with high spatial resolution and can be an important tool for providing information between the disparate scales of clinical and pathological imaging.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mp.17336","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T20:15:56Z","timestamp":1722543356000},"page":"9179-9193","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phase\u2010contrast visualization of human tissues using superimposed wavefront imaging of diffraction\u2010enhanced 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