{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T15:48:27Z","timestamp":1777477707260,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,5,19]],"date-time":"2002-05-19T00:00:00Z","timestamp":1021766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Med. Pediatr. Oncol."],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The classification of B\u2010lineage acute lymphoblastic leukemia (ALL) by specific chromosomal translocations may have prognostic implications. Reverse transcriptase\u2010polymerase chain reaction (RT\u2010PCR) assay is a useful tool for the detection of fusion transcript resulting from specific chromosomal translocation of the leukemic cells. In general, fusion transcripts are determined individually, a process which is labor intensive in order to detect all major fusion transcripts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>We use a multiplex RT\u2010PCR assay to detect both the CML\u2010 and ALL\u2010type <jats:italic>BCR\u2010ABL<\/jats:italic> transcripts of the t(9;22), all described variants of the <jats:italic>E2A\u2010PBX1<\/jats:italic> transcripts of t(1;19), the <jats:italic>MLL\u2010AF4<\/jats:italic> transcripts of t(4;11), and all described variants of <jats:italic>TEL\u2010AML1<\/jats:italic> (also termed <jats:italic>ETV6\u2010CBFA2<\/jats:italic>) of the cryptic t(12;21) in 165 leukemic samples at diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The study yielded a completely concordant result with those obtained by the individual RT\u2010PCR assay. In this cohort of Taiwan children, the relative frequencies of the four translocations of B\u2010lineage ALL were as following: 6% with ALL\u2010type t(9;22)\/<jats:italic>BCR\u2010ABL<\/jats:italic>, 7% t(1;19)\/<jats:italic>E2A\u2010PBX1<\/jats:italic>, 3% t(4;11)\/<jats:italic>MLL\u2010AF4<\/jats:italic>, and 18% t(12;21)\/<jats:italic>TEL\u2010AML1<\/jats:italic>, comparable to those in the Western countries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Multiplex RT\u2010PCR assay is an efficient, sensitive, accurate, and cost\u2010effective diagnostic tool, which will likely improve our ability in accurately and rapidly risk\u2010stratifying children with ALL. Med Pediatr Oncol 2002;39:12\u201317. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mpo.10092","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:48:25Z","timestamp":1030319305000},"page":"12-17","source":"Crossref","is-referenced-by-count":22,"title":["Multiplex RT\u2010PCR assay for the detection of major fusion transcripts in Taiwanese children with B\u2010lineage acute lymphoblastic leukemia"],"prefix":"10.1002","volume":"39","author":[{"given":"Der\u2010Cherng","family":"Liang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee\u2010Yung","family":"Shih","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao\u2010Ping","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iou\u2010Jih","family":"Hung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu\u2010Huey","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tang\u2010Her","family":"Jaing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsi\u2010Che","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan\u2010Hui","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,5,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199506153322407"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V77.3.440.440"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V76.3.489.489"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V76.1.117.117"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1991.9.12.2183"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V86.11.4263.bloodjournal86114263"},{"key":"e_1_2_1_8_2","first-page":"1985","article-title":"TEL\/AML1 fusion resulting from a cryptic t(12; 21) is the most common genetic lesion in pediatric ALL and defines a subgroup of patients with an excellent prognosis","volume":"9","author":"Shurtleff SA","year":"1995","journal-title":"Leukemia"},{"key":"e_1_2_1_9_2","first-page":"991","article-title":"High incidence of TEL\/AML1 fusion resulting from a cryptic t(12;21) in childhood B\u2010lineage acute lymphoblastic leukemia in Taiwan","volume":"10","author":"Liang D\u2010C","year":"1996","journal-title":"Leukemia"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.11.4252.bloodjournal88114252"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1997.15.3.1150"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.4.1143"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1182\/blood.V90.2.571","article-title":"Incidence and clinical relevance of TEL\/AML1 fusion genes in children with acute lymphoblastic leukemia enrolled in the German and Italian multicenter therapy trials. 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