{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:52:09Z","timestamp":1787237529316,"version":"build-2736575974"},"reference-count":14,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,1,21]],"date-time":"2002-01-21T00:00:00Z","timestamp":1011571200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Med. Pediatr. Oncol."],"published-print":{"date-parts":[[2002,2]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n During recent years, we identified characteristic genetic alterations in sporadic hepatoblastoma (HB). These include loss of heterozygosity (LOH) at the chromosomal region 11p15.5, LOH on chromosome arms 1p and 1q, activating mutations in exon 3 of the \u03b2\u2010\n catenin<\/jats:italic>\n gene, as well as overexpression of the\n c\u2010met<\/jats:italic>\n oncogene, which encodes the hepatocyte growth factor receptor. We now wanted to evaluate the prognostic relevance of these abnormalities concerning the outcome in a large group of patients.\n <\/jats:p>\n <\/jats:sec>\n \n Procedure<\/jats:title>\n \n All but 7 of 56 patients with HB were treated either with primary resection for small tumors, or neo\u2010adjuvant chemotherapy with ifosfamide, cisplatin and doxorubicin (IPA) before delayed surgery in case of extended disease and additional adjuvant IPA therapy in all cases. Seven tumors were primarily resected and adjuvantly treated with different cytotoxic drugs. LOH 11p15.5, LOH 1p, and LOH 1q were detected in tumor tissue in comparison to normal liver and\/or peripheral blood leukocytes by PCR based microsatellite analysis. Beta\u2010\n catenin<\/jats:italic>\n mutations were analysed with SSCP, deletion screening and direct sequencing. RT\u2010PCR was used for identification of\n c\u2010met<\/jats:italic>\n mRNA overexpression. The results were correlated with the tumors' stage, histological type and epithelial differentiation, as well as with the patients' outcome.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Overall disease\u2010free survival after median follow\u2010up of 5 years was 43\/56 patients (77%). LOH 11p15.5 was found in 15\/56, LOH1p in 11\/53, LOH1q in 7\/53 and \u03b2\u2010\n catenin<\/jats:italic>\n mutations in 25\/55 HB. 13\/23 HB had a\n c\u2010met<\/jats:italic>\n overexpression. LOH 11p15.5 and LOH 1p were significantly more often found in embryonal HB, while there was no correlation of other genetic alterations with histological type or differentiation. Furthermore, statistical analysis revealed no correlation of any of these disorders with initial tumor stage, nor with the patients' outcome.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n None of the investigated molecular genetic alterations are suited to serve as a prognostic indicator in HB. Further investigations, especially genetic screening of tumor tissue may reveal prognostic markers for this neoplasm. Med Pediatr Oncol 2002;38:104\u2013108. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/mpo.1280","type":"journal-article","created":{"date-parts":[[2004,10,25]],"date-time":"2004-10-25T11:34:20Z","timestamp":1098704060000},"page":"104-108","source":"Crossref","is-referenced-by-count":28,"title":["Prognostic impact of molecular genetic alterations in hepatoblastoma"],"prefix":"10.1002","volume":"38","author":[{"given":"Dietrich","family":"von Schweinitz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen A.","family":"Kraus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Albrecht","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arend","family":"Koch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Fuchs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torsten","family":"Pietsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,1,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(83)80005-7"},{"key":"e_1_2_1_3_2","first-page":"1243","article-title":"Hepatoblastoma\u2014a bit of a success story?","volume":"33","author":"Shafford EA","year":"1997","journal-title":"Eur J Cancer"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19971219)74:6<593::AID-IJC6>3.0.CO;2-P"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(00)00074-5"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(97)00432-9"},{"key":"e_1_2_1_7_2","first-page":"5041","article-title":"Loss of maternal alleles on chromosome arm 11p in hepatoblastoma","volume":"54","author":"Albrecht S","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19960807)67:4<467::AID-IJC1>3.0.CO;2-Q"},{"key":"e_1_2_1_9_2","first-page":"269","article-title":"Childhood hepatoblastomas frequently carry a mutated degradation targeting box of the \u03b2\u2010catenin gene","volume":"59","author":"Koch A","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(20000115)85:2<151::AID-IJC1>3.0.CO;2-6"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(97)00095-6"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1043805"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6719137"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(200002)27:2<196::AID-GCC12>3.0.CO;2-D"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64567-1"}],"container-title":["Medical and Pediatric Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmpo.1280","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mpo.1280","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T06:51:12Z","timestamp":1700376672000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mpo.1280"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1,21]]},"references-count":14,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,2]]}},"alternative-id":["10.1002\/mpo.1280"],"URL":"https:\/\/doi.org\/10.1002\/mpo.1280","archive":["Portico"],"relation":{},"ISSN":["0098-1532","1096-911X"],"issn-type":[{"value":"0098-1532","type":"print"},{"value":"1096-911X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,1,21]]}}}