{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T05:04:17Z","timestamp":1715231057492},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T00:00:00Z","timestamp":1153353600000},"content-version":"vor","delay-in-days":4583,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Med. Pediatr. Oncol."],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new high\u2010performance liquid chromatographic assay was used to determine methotrexate (MTX) and its main metabolite, 7\u2010hydroxymethotrexate (7\u2010OH\u2010MTX), in the plasma (n \u2010 17) and urine (n = 14) of children (age 3\u201012 years) on maintenance therapy for acute lymphocytic leukemia (n = 14) or non\u2010Hodgkin's lymphoma (n = 3). Each child received oral doses of weekly MTX (4.0\u201029 mg\/m<jats:sup>2<\/jats:sup>) and daily 6\u2010mer\u2010captopurine (40\u2010111 mg\/m<jats:sup>2<\/jats:sup>). Plasma samples were collected daily from two children during the 1\u2010week dose interval. A limited sampling strategy was designed, whereby 2 days of blood sampling were used in the other 15 patients. Morning urine samples were collected daily for 1 week following MTX intake from 14 of the children. MTX was detectable in all plasma and urine samples for the entire dose interval. The main metabolite, 7\u2010OH\u2010MTX, could be detected in plasma and urine from all patients on the first day after dose intake but only in a few patients during the whole dose interval. Interpatient variability of MTX and 7\u2010OH\u2010MTX levels was high at all points during the week. Significant correlation were found between the urinary MTX levels on days 2 and 7 and plasma MTX levels on day 2 after intake. No significant correlation was found between drug levels in plasma or urine and liver function tests in the children showing signs of mild liver injury. This assay provides a tool for further studies on the role of pharmacokinetics for the clinical effects of weekly oral low\u2010dose MTX given alone or in combination with 6\u2010mercaptopurine. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mpo.2950220307","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T19:14:08Z","timestamp":1171998848000},"page":"187-193","source":"Crossref","is-referenced-by-count":8,"title":["Plasma and urine levels of methotrexate and 7\u2010hydroxymethotrexate in children with all during maintenance therapy with weekly oral methotrexate"],"prefix":"10.1002","volume":"22","author":[{"given":"Kathleen A.","family":"Skoglund","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"S\u00f6derh\u00e4ll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olof","family":"Beck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Curt","family":"Peterson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mona","family":"Wennberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salah","family":"Hayder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olle","family":"Bj\u00f6rk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM194806032382301"},{"key":"e_1_2_1_3_2","first-page":"110","volume-title":"Cancer Chemotherapy: Principles and Practice","author":"Allegra CJ","year":"1990"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197412052912306"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197508)36:2<341::AID-CNCR2820360208>3.0.CO;2-3"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.1986.tb10293.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198304283081705"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.3109\/08880018609031198"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-198911000-00001"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199007053230104"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1989.7.12.1816"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","unstructured":"SchmiegelowK Schr\u00f6derH GlomsteinA SalmiT SiimesM Wranne LKristinssonJ:Prognostic significance of red blood cell concentrations of cytotoxic metabolites of MTX and 6\u2010MP during maintenance chemotherapy for childhood acute lymphoblastic leukemia. SIOP XXIII Mtg Abs no 307 1991.","DOI":"10.3109\/08880019109028803"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/mpo.2950180310"},{"key":"e_1_2_1_14_2","first-page":"1","article-title":"A specific HPLC assay to determine the pharmacokinetics of methotrexate in patients","volume":"22","author":"Chen M","year":"1984","journal-title":"Int J Clin Pharmacol Ther Toxicol"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00262278"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(87)80025-2"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1991.263"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)90411-2"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-199111000-00011"},{"key":"e_1_2_1_20_2","first-page":"507","volume-title":"Gradwohl's Clinical Laboratory Methods and Diagnosis","author":"McNeely MDD","year":"1980"},{"key":"e_1_2_1_21_2","first-page":"22","article-title":"In vivo methotrexate kinetics and metabolism in human hematopoietic cells","volume":"37","author":"Schr\u00f8der H","year":"1990","journal-title":"Dan Med Bull"},{"key":"e_1_2_1_22_2","first-page":"530","article-title":"Pharmacokinetics in blood. IV. Unusual distribution, storage effect and metabolism of methotrexate","volume":"22","author":"Lee MG","year":"1984","journal-title":"Int J Clin Pharmacol Ther Toxicol"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1988.tb05149.x"}],"container-title":["Medical and Pediatric Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmpo.2950220307","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mpo.2950220307","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T12:29:37Z","timestamp":1698150577000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mpo.2950220307"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1994,1]]}},"alternative-id":["10.1002\/mpo.2950220307"],"URL":"https:\/\/doi.org\/10.1002\/mpo.2950220307","archive":["Portico"],"relation":{},"ISSN":["0098-1532","1096-911X"],"issn-type":[{"value":"0098-1532","type":"print"},{"value":"1096-911X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,1]]}}}