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There has yet to be an investigation however on the effects of mood\u2010stabilizers on housekeeping gene stability. The current study utilized lymphoblastoid cell lines (LCLs) derived from patients with mood disorders to investigate the effects of a range of doses of lithium (0, 1, 2 and 5\u2009mM) and sodium valproate (0, 0.06, 0.03 and 0.6\u2009mM) on the stability of 12 housekeeping genes. RNA was extracted from LCLs and qPCR was used to generate cycle threshold (<jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub>) values which were input into RefFinder analyses. The study revealed drug\u2010specific effects on housekeeping gene stability. The most stable housekeeping genes in LCLs treated: acutely with sodium valproate were<jats:italic>ACTB<\/jats:italic>and<jats:italic>RPL13A;<\/jats:italic>acutely with lithium were<jats:italic>GAPDH<\/jats:italic>and<jats:italic>ATP5B<\/jats:italic>; chronically with lithium were<jats:italic>ATP5B<\/jats:italic>and<jats:italic>CYC1<\/jats:italic>. The stability of<jats:italic>GAPDH<\/jats:italic>and<jats:italic>B2M<\/jats:italic>were particularly affected by duration of lithium treatment. The study adds to a growing literature that the selection of appropriate housekeeping genes is important for the accurate normalization of target gene expression in experiments investigating the molecular effects of mood disorder pharmacotherapies.<jats:italic>Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:italic><\/jats:p>","DOI":"10.1002\/mpr.1435","type":"journal-article","created":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T12:35:41Z","timestamp":1392208541000},"page":"279-288","source":"Crossref","is-referenced-by-count":16,"title":["Mood\u2010stabilizers differentially affect housekeeping gene expression in human cells"],"prefix":"10.1002","volume":"23","author":[{"given":"Timothy R.","family":"Powell","sequence":"first","affiliation":[{"name":"King's College London, MRC Social Genetic and Developmental Psychiatry (SGDP) Centre Institute of Psychiatry London 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Translational Medicine University of Liverpool Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard C.","family":"Schalkwyk","sequence":"additional","affiliation":[{"name":"King's College London, MRC Social Genetic and Developmental Psychiatry (SGDP) Centre Institute of Psychiatry London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne E.","family":"Farmer","sequence":"additional","affiliation":[{"name":"King's College London, MRC Social Genetic and Developmental Psychiatry (SGDP) Centre Institute of Psychiatry London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula M.","family":"D'Souza","sequence":"additional","affiliation":[{"name":"King's College London, MRC Social Genetic and Developmental Psychiatry (SGDP) Centre Institute of Psychiatry London 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