{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T11:09:18Z","timestamp":1790939358486,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,4,6]],"date-time":"2005-04-06T00:00:00Z","timestamp":1112745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Magnetic Reson in Chemistry"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The usefulness of bovine serum albumin (BSA) as a model protein for testing NMR methods for the study of protein\u2013ligand interactions is discussed. Isothermal titration calorimetry established the binding affinity and stoichiometry of the specific binding site for <jats:sc>L<\/jats:sc>\u2010tryptophan, <jats:sc>D<\/jats:sc>\u2010tryptophan, naproxen, ibuprofen, salicylic acid and warfarin. The binding affinities of the same ligands determined by NMR methods are universally weaker (larger <jats:italic>K<\/jats:italic><jats:sub>D<\/jats:sub>). This is because the NMR methods are susceptible to interference from additional non\u2010specific binding. The <jats:sc>L<\/jats:sc>\u2010tryptophan\u2013BSA and naproxen\u2013BSA systems were the best behaved model systems. Copyright \u00a9 2005 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/mrc.1574","type":"journal-article","created":{"date-parts":[[2005,4,6]],"date-time":"2005-04-06T10:48:04Z","timestamp":1112784484000},"page":"463-470","source":"Crossref","is-referenced-by-count":119,"title":["Determination of protein\u2013ligand binding affinity by NMR: observations from serum albumin model systems"],"prefix":"10.1002","volume":"43","author":[{"given":"Lee","family":"Fielding","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Rutherford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Fletcher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,4,6]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnmrs.2004.03.001"},{"key":"e_1_2_1_3_2","volume-title":"All About Albumin","author":"Peters T","year":"1996"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/S0026-895X(25)10691-3","volume":"11","author":"Sudlow G","year":"1975","journal-title":"Mol. 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