{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:33:15Z","timestamp":1761006795615,"version":"build-2065373602"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,8,4]],"date-time":"2013-08-04T00:00:00Z","timestamp":1375574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Magnetic Reson in Chemistry"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:p>Phosphorylation and dephosphorylation of starch and glycogen are important for their physicochemical properties and also their physiological functions. It is therefore desirable to reliably determine the phosphorylation sites. Heteronuclear multidimensional NMR\u2010spectroscopy is in principle a straightforward analytical approach even for complex carbohydrate molecules. With heterogeneous samples from natural sources, however, the task becomes more difficult because a full assignment of the resonances of the carbohydrates is impossible to obtain. Here, we show that the combination of heteronuclear <jats:sup>1<\/jats:sup>H,<jats:sup>13<\/jats:sup>C and <jats:sup>1<\/jats:sup>H,<jats:sup>13<\/jats:sup>C,<jats:sup>31<\/jats:sup>P techniques and information derived from spectra of a set of reference compounds can lead to an unambiguous determination of the phosphorylation sites even in heterogeneous samples. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/mrc.3996","type":"journal-article","created":{"date-parts":[[2013,8,5]],"date-time":"2013-08-05T00:48:27Z","timestamp":1375663707000},"page":"655-661","source":"Crossref","is-referenced-by-count":6,"title":["Determination of glucan phosphorylation using heteronuclear <sup>1<\/sup>H,<sup>13<\/sup>C double and <sup>1<\/sup>H,<sup>13<\/sup>C,<sup>31<\/sup>P triple\u2010resonance NMR spectra"],"prefix":"10.1002","volume":"51","author":[{"given":"Peter","family":"Schmieder","sequence":"first","affiliation":[{"name":"Leibniz\u2010Institut f\u00fcr Molekulare Pharmakologie (FMP)  Robert\u2010R\u00f6ssle\u2010Str. 10 D\u201013125 Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix","family":"Nitschke","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Biology, Plant Physiology University of Potsdam  Karl\u2010Liebknecht\u2010Str. 24\u201025 Building 20 D\u201014476 Potsdam\u2010Golm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Steup","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Biology, Plant Physiology University of Potsdam  Karl\u2010Liebknecht\u2010Str. 24\u201025 Building 20 D\u201014476 Potsdam\u2010Golm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keven","family":"Mallow","sequence":"additional","affiliation":[{"name":"Leibniz\u2010Institut f\u00fcr Molekulare Pharmakologie (FMP)  Robert\u2010R\u00f6ssle\u2010Str. 10 D\u201013125 Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgar","family":"Specker","sequence":"additional","affiliation":[{"name":"Leibniz\u2010Institut f\u00fcr Molekulare Pharmakologie (FMP)  Robert\u2010R\u00f6ssle\u2010Str. 10 D\u201013125 Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,8,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erq411"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/star.201000013"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2950477"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(99)76982-1"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-042809-112301"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20111416"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2013.04.006"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200704094"},{"key":"e_1_2_6_10_1","first-page":"279","volume-title":"Starch: Origins, Structure and Metabolism","author":"Hejazi M.","year":"2012"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M807428200"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(80)81317-2"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)80234-L"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2011.01.017"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.07.085"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-1988-27494"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(00)86062-1"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.062053099"},{"key":"e_1_2_6_19_1","first-page":"509","volume":"11","author":"Zmudzka B.","year":"1964","journal-title":"Acta Biochim. Pol."},{"volume-title":"SPARKY 3","author":"Goddard T. D.","key":"e_1_2_6_20_1"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00388a062"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00362a038"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(86)90091-0"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(89)90153-4"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00268a061"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00308a042"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.451421"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(87)90102-8"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200300700"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/star.19710230803"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-8130(00)00121-5"},{"key":"e_1_2_6_32_1","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/S0021-9258(18)56173-1","volume":"188","author":"Posternak T.","year":"1951","journal-title":"J. Biol. Chem."},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(85)90122-2"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(86)90395-1"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(89)90152-2"}],"container-title":["Magnetic Resonance in Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrc.3996","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrc.3996","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrc.3996","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:06:27Z","timestamp":1760954787000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/mrc.3996"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,4]]},"references-count":34,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["10.1002\/mrc.3996"],"URL":"https:\/\/doi.org\/10.1002\/mrc.3996","archive":["Portico"],"relation":{},"ISSN":["0749-1581","1097-458X"],"issn-type":[{"type":"print","value":"0749-1581"},{"type":"electronic","value":"1097-458X"}],"subject":[],"published":{"date-parts":[[2013,8,4]]}}}