{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T00:23:41Z","timestamp":1774398221305,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,7,10]],"date-time":"2018-07-10T00:00:00Z","timestamp":1531180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","award":["EMR\/2016\/001756"],"award-info":[{"award-number":["EMR\/2016\/001756"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Reson in Chemistry"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Currently, there are no reliable biomarkers available that can aid early differential diagnosis of reactive arthritis (ReA) from other inflammatory joint diseases. Metabolic profiling of synovial fluid (SF)\u2014obtained from joints affected in ReA\u2014holds great promise in this regard and will further aid monitoring treatment and improving our understanding about disease mechanism. As a first step in this direction, we report here the metabolite specific assignment of <jats:sup>1<\/jats:sup>H and <jats:sup>13<\/jats:sup>C resonances detected in the NMR spectra of SF samples extracted from human patients with established ReA. The metabolite characterization has been carried out on both normal and ultrafiltered (deproteinized) SF samples of eight ReA patients (<jats:italic>n<\/jats:italic>\u00a0=\u00a08) using high\u2010resolution (800\u00a0MHz) <jats:sup>1<\/jats:sup>H and <jats:sup>1<\/jats:sup>H\u2500<jats:sup>13<\/jats:sup>C NMR spectroscopy methods such as one\u2010dimensional <jats:sup>1<\/jats:sup>H CPMG and two\u2010dimensional J\u2010resolved<jats:sup>1<\/jats:sup>H NMR and homonuclear <jats:sup>1<\/jats:sup>H\u2500<jats:sup>1<\/jats:sup>H TOCSY and heteronuclear<jats:sup>1<\/jats:sup>H\u2500<jats:sup>13<\/jats:sup>C HSQC correlation spectra. Compared with normal SF samples, several distinctive <jats:sup>1<\/jats:sup>H NMR signals were identified and assigned to metabolites in the <jats:sup>1<\/jats:sup>H NMR spectra of ultrafiltered SF samples. Overall, we assigned 53 metabolites in normal filtered SF and 64 metabolites in filtered pooled SF sample compared with nonfiltered SF samples for which only 48 metabolites (including lipid\/membrane metabolites as well) have been identified. The established NMR characterization of SF metabolites will serve to guide future metabolomics studies aiming to identify\/evaluate the SF\u2010based metabolic biomarkers of diagnostic\/prognostic potential or seeking biochemical insights into disease mechanisms in a clinical perspective.<\/jats:p>","DOI":"10.1002\/mrc.4763","type":"journal-article","created":{"date-parts":[[2018,6,15]],"date-time":"2018-06-15T21:29:41Z","timestamp":1529098181000},"page":"30-43","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Metabolite assignment of ultrafiltered synovial fluid extracted from knee joints of reactive arthritis patients using high resolution <scp>NMR<\/scp> spectroscopy"],"prefix":"10.1002","volume":"57","author":[{"given":"Durgesh","family":"Dubey","sequence":"first","affiliation":[{"name":"Centre of Biomedical Research Sanjay Gandhi Postgraduate Institute of Medical Sciences  Lucknow India"},{"name":"Babasaheb Bhimrao Ambedkar University  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Smriti","family":"Chaurasia","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Sanjay Gandhi Postgraduate Institute of Medical Sciences  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4158-2241","authenticated-orcid":false,"given":"Anupam","family":"Guleria","sequence":"additional","affiliation":[{"name":"Centre of Biomedical Research Sanjay Gandhi Postgraduate Institute of Medical Sciences  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandeep","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Sanjay Gandhi Postgraduate Institute of Medical Sciences  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dinesh Raj","family":"Modi","sequence":"additional","affiliation":[{"name":"Babasaheb Bhimrao Ambedkar University  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7921-4199","authenticated-orcid":false,"given":"Ramnath","family":"Misra","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Sanjay Gandhi Postgraduate Institute of Medical Sciences  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8079-6739","authenticated-orcid":false,"given":"Dinesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Centre of Biomedical Research Sanjay Gandhi Postgraduate Institute of Medical Sciences  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,7,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199809)186:1<75::AID-PATH142>3.0.CO;2-B"},{"issue":"1","key":"e_1_2_7_4_1","first-page":"115","volume":"21","author":"Kvien T. 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