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Its molecular structure was determined using 1D and 2D NMR spectroscopic techniques (\n                    <jats:sup>1<\/jats:sup>\n                    H,\n                    <jats:sup>13<\/jats:sup>\n                    C, HSQC, COSY, HMBC, and TOCSY) and MALDI\u2010TOF mass spectrometry. Additionally, the antiprotozoal activity was evaluated. Papaucin (\n                    <jats:bold>1<\/jats:bold>\n                    ) showed moderate positive activity compared with in\u00a0vitro assays of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Entamoeba histolytica<\/jats:italic>\n                    <\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Giardia lamblia<\/jats:italic>\n                    <\/jats:styled-content>\n                    , with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 63.07 and 67.62\u2009\u03bcg\u2009mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    respectively. On the other hand, in silico studies revealed a higher affinity of papaucin for the PFOR enzyme compared with metronidazole, as evidenced by a greater number of polar interactions. Regarding the ALR enzyme, papaucin also showed affinity, although at a binding site different from the main catalytic site. These results suggest that papaucin could exert its antiprotozoal effect through multiple mechanisms.\n                  <\/jats:p>","DOI":"10.1002\/mrc.70065","type":"journal-article","created":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T01:00:02Z","timestamp":1764032402000},"page":"217-226","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Structural Elucidation of the Cyclopeptide Papaucin Isolated From\n                    <scp>\n                      <i>Annona diversifolia<\/i>\n                    <\/scp>\n                    Saff. Seeds by NMR, Antiprotozoal Activity and Molecular Docking Studies"],"prefix":"10.1002","volume":"64","author":[{"given":"Ulises","family":"Murrieta\u2010Dionicio","sequence":"first","affiliation":[{"name":"Laboratorio de Productos Naturales, \u00c1rea de Qu\u00edmica, Departamento de Preparatoria Agr\u00edcola Universidad Aut\u00f3noma Chapingo  Chapingo M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Calzada","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Farmacolog\u00eda, UMAE Hospital de Especialidades, 2\u00b0 Piso CORSE Centro M\u00e9dico Nacional Siglo XXI, IMSS  Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional  Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7401-303X","authenticated-orcid":false,"given":"Miguel","family":"Vald\u00e9s","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Farmacolog\u00eda, UMAE Hospital de Especialidades, 2\u00b0 Piso CORSE Centro M\u00e9dico Nacional Siglo XXI, IMSS  Ciudad de M\u00e9xico M\u00e9xico"},{"name":"Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional  Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benito","family":"Reyes\u2010Trejo","sequence":"additional","affiliation":[{"name":"Laboratorio de Productos Naturales, \u00c1rea de Qu\u00edmica, Departamento de Preparatoria Agr\u00edcola Universidad Aut\u00f3noma Chapingo  Chapingo M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holber","family":"Zuleta\u2010Prada","sequence":"additional","affiliation":[{"name":"Laboratorio de Productos Naturales, \u00c1rea de Qu\u00edmica, Departamento de Preparatoria Agr\u00edcola Universidad Aut\u00f3noma Chapingo  Chapingo M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lino","family":"Reyes","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Org\u00e1nica, Facultad de Qu\u00edmica Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad Universitaria  Delegaci\u00f3n Coyoac\u00e1n M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"del R\u00edo\u2010Portilla","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico  Ciudad de M\u00e9xico 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