{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T02:04:09Z","timestamp":1789610649076,"version":"build-2803163510"},"reference-count":24,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["AEI\/10.13039\/501100011033\/PID2022\u2010142879NB\u2010I00"],"award-info":[{"award-number":["AEI\/10.13039\/501100011033\/PID2022\u2010142879NB\u2010I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Reson in Chemistry"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Saturation transfer difference nuclear magnetic resonance (STD NMR) spectroscopy is a powerful and robust technique for the characterization of receptor\u2013ligand interactions of medium to weak affinity in solution, providing binding epitope maps that reveal structural details of these interactions at atomic level. This knowledge is essential for understanding the structural basis of biomolecular recognition processes of biological relevance and plays a key role in drug discovery. Temperature is a critical factor influencing the sensitivity of STD NMR spectroscopy, as it directly affects the kinetics of complex formation, thereby modulating the observed STD effects. In this study, we systematically investigated the impact of temperature on STD NMR\u2010derived binding epitope maps. Our findings highlight that, whenever protein stability permits, temperature should be considered an essential experimental parameter to be optimized for each system to obtain binding epitope maps with well\u2010discriminated protein\u2013ligand contact information. For the example cases studied herein, we demonstrate that temperature optimization allows to better assess the contribution of each individual contact to protein\u2013ligand binding (enhanced ligand epitope mapping discrimination).<\/jats:p>","DOI":"10.1002\/mrc.70141","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T01:34:46Z","timestamp":1789608886000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhancing Ligand Epitope Mapping in Biomolecular Recognition by Variable Temperature Saturation Transfer Difference NMR (VT\u2010STD NMR)"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5673-5943","authenticated-orcid":false,"given":"Gabriel","family":"Rocha","sequence":"first","affiliation":[{"name":"Biomolecular Interactions and Structural Glycobiology Group Institute for Chemical Research (IIQ), CSIC \u2013 University of Seville  Seville Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4106-299X","authenticated-orcid":false,"given":"Svetla","family":"Daskalova","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry and Pharmacy University of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2246-3236","authenticated-orcid":false,"given":"Juan C.","family":"Mu\u00f1oz\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Biomolecular Interactions and Structural Glycobiology Group Institute for Chemical Research (IIQ), CSIC \u2013 University of Seville  Seville Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7250-5639","authenticated-orcid":false,"given":"Jes\u00fas","family":"Angulo","sequence":"additional","affiliation":[{"name":"Biomolecular Interactions and Structural Glycobiology Group Institute for Chemical Research (IIQ), CSIC \u2013 University of Seville  Seville Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1007\/s00249-011-0749-5","article-title":"STD\u2010NMR: Application to Transient Interactions Between Biomolecules\u2014A Quantitative Approach","volume":"40","author":"Angulo J.","year":"2011","journal-title":"European Biophysics Journal"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1039\/D2CS00983H","article-title":"Exploring Multivalent Carbohydrate\u2013Protein Interactions by NMR","volume":"52","author":"Quintana J. 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