{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T07:41:30Z","timestamp":1792568490852,"version":"4.5.0"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Reson in Chemistry"],"abstract":"ABSTRACT<\/jats:title>\n \n Antimicrobial resistance (AMR) remains one of the greatest global public health challenges of the 21st century, compromising the effectiveness of current therapies and highlighting the urgent need for new antibacterial agents. Natural products from bacteria and filamentous fungi continue to represent an invaluable source of structurally diverse bioactive metabolites. In this study, a library of 56 extracts obtained from mangrove\u2010associated ascomycetes was screened against a panel of multidrug\u2010resistant bacterial pathogens. Bioactivity data were integrated with LC\u2013MS\/MS\u2010based molecular networking to prioritize extracts for chemical investigation. This approach identified\n Fusarium<\/jats:italic>\n sp. IQ\u20101921 as one of the most promising candidates, leading to the isolation of a new dihydroisocoumarin (\n 1<\/jats:bold>\n ) and four known analogues (\n 2<\/jats:bold>\n \u2013\n 5<\/jats:bold>\n ). The structures of all isolated metabolites were elucidated by 1D and 2D NMR spectroscopy and high\u2010resolution mass spectrometry (HRMS). All compounds were evaluated against multidrug\u2010resistant strains of\n \n Acinetobacter baumannii<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n Pseudomonas aeruginosa<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n Klebsiella pneumoniae<\/jats:italic>\n <\/jats:styled-content>\n , and\n \n Staphylococcus aureus<\/jats:italic>\n <\/jats:styled-content>\n . Compounds\n 1<\/jats:bold>\n and\n 3<\/jats:bold>\n \u2013\n 5<\/jats:bold>\n exhibited moderate antibacterial activity, with compound\n 1<\/jats:bold>\n displaying the broadest inhibitory profile. These findings demonstrate the effectiveness of integrating metabolomics with bioactivity\u2010guided prioritization for the discovery of antibacterial natural products and further highlight the indispensable role of NMR spectroscopy in the structural elucidation of closely related fungal metabolites.\n <\/jats:p>","DOI":"10.1002\/mrc.70146","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T07:08:11Z","timestamp":1792566491000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["NMR\u2010Based Structural Elucidation of a New Dihydroisocoumarin and Related Polyketides From a Mangrove\u2010Derived\n \n Fusarium<\/i>\n <\/scp>\n sp."],"prefix":"10.1002","author":[{"given":"Evelyn","family":"Garc\u00eda\u2010Oceguera","sequence":"first","affiliation":[{"name":"Department of Natural Products, Instituto de Qu\u00edmica Universidad Nacional Aut\u00f3noma de M\u00e9xico Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos A.","family":"Fajardo\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Natural Products, Instituto de Qu\u00edmica Universidad Nacional Aut\u00f3noma de M\u00e9xico Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ixchel A.","family":"Loa\u2010Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Department of Natural Products, Instituto de Qu\u00edmica Universidad Nacional Aut\u00f3noma de M\u00e9xico Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9010-6268","authenticated-orcid":false,"given":"Jes\u00fas I.","family":"Morales\u2010Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, Experimental Hematology Laboratory Universidad Aut\u00f3noma Metropolitana, Iztapalapa Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9225-6200","authenticated-orcid":false,"given":"Jos\u00e9","family":"Rivera\u2010Ch\u00e1vez","sequence":"additional","affiliation":[{"name":"Department of Natural Products, Instituto de Qu\u00edmica Universidad Nacional Aut\u00f3noma de M\u00e9xico Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,21]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.lanmic.2024.07.010","article-title":"Antimicrobial Resistance: A Concise Update","volume":"6","author":"Ho C. 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V.","year":"2024","journal-title":"Journal of the Brazilian Chemical Society"},{"key":"e_1_2_10_9_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/978-981-96-2602-1_3","volume-title":"Mangrove Microbiome: Diversity and Bioprospecting","author":"Pattapulavar V.","year":"2025"},{"issue":"12","key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.1039\/D0NP00038H","article-title":"Fusarium: A Treasure Trove of Bioactive Secondary Metabolites","volume":"37","author":"Li M.","year":"2020","journal-title":"Natural Product Reports"},{"issue":"2","key":"e_1_2_10_11_1","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1039\/D2RA04126J","article-title":"The Endophytic Fusarium Strains: A Treasure Trove of Natural Products","volume":"13","author":"Ahmed A. 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