{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T03:11:41Z","timestamp":1767841901677,"version":"3.49.0"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,6,13]],"date-time":"2002-06-13T00:00:00Z","timestamp":1023926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Membrane cofactor protein (MCP; CD46) is a complement regulator widely expressed as four isoforms that arise via alternative splicing. On human spermatozoa, MCP is expressed on the inner acrosomal membrane and alterations of spermatozoa MCP may be associated with infertility. In rodents, expression of MCP is largely restricted to the testes. MCP on human spermatozoa has a unique M<jats:sub>r<\/jats:sub> pattern that we have investigated. We also characterized MCP expression in mice transgenic (tg) for human MCP. Human MCP expression in the tg mice mimics the human pattern in that it is located on the inner acrosomal membrane and has a faster M<jats:sub>r<\/jats:sub> than MCP expressed elsewhere. Sequencing of RT\u2010PCR products from the testis indicates that there is not a unique male reproductive tissue specific cytoplasmic tail. Instead, human spermatozoa express MCP bearing cytoplasmic tail two, which is also utilized in most other tissues and contains several signaling motifs. Further, using <jats:italic>N<\/jats:italic>\u2010glycosidases, we demonstrate that the unique lower molecular weight of MCP on spermatozoa is secondary to a modification in the <jats:italic>N<\/jats:italic>\u2010linked sugars. Specifically, as the spermatozoa mature, but before they reach the epididymis, the three <jats:italic>N<\/jats:italic>\u2010linked sugars of MCP are trimmed to less complex structures. While the purpose of this deglycosylation is unknown, we propose that it is a common feature of proteins expressed on the plasma and inner acrosomal membranes of spermatozoa and hypothesize that it is a spermatozoa specific event critical for facilitating sperm\u2013egg interactions. Mol. Reprod. Dev. 62:534\u2013546, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.10144","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T12:38:48Z","timestamp":1033907928000},"page":"534-546","source":"Crossref","is-referenced-by-count":43,"title":["Characterization of human membrane cofactor protein (MCP; CD46) on spermatozoa"],"prefix":"10.1002","volume":"62","author":[{"given":"Rebecca C.","family":"Riley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Kemper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilyn","family":"Leung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John P.","family":"Atkinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,6,13]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.147.9.3005"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080290313"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod41.2.285"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.21.10051"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-1809.1985.tb01673.x"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"3850","DOI":"10.4049\/jimmunol.138.11.3850","article-title":"A polymorphism of the complement regulatory protein MCP (membrane cofactor protein or gp 45\u201370)","volume":"138","author":"Ballard L","year":"1987","journal-title":"J Immunol"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"3923","DOI":"10.4049\/jimmunol.141.11.3923","article-title":"Biochemical characterization of membrane cofactor protein of the complement system","volume":"141","author":"Ballard LL","year":"1988","journal-title":"J Immunol"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"2821","DOI":"10.4049\/jimmunol.146.8.2821","article-title":"Membrane cofactor protein of the complement system: A Hind III restriction fragment length polymorphism that correlates with the expression polymorphism","volume":"146","author":"Bora NS","year":"1991","journal-title":"J Immunol"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2605.2000.00027.x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1996.0122"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.4049\/jimmunol.148.5.1431","article-title":"Identification and characterization of membrane cofactor protein (MCP) on human spermatozoa","volume":"148","author":"Cervoni F","year":"1992","journal-title":"J Immunol"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"939","DOI":"10.4049\/jimmunol.151.2.939","article-title":"Expression of decay accelerating factor (CD55) of the complement system on human spermatozoa","volume":"151","author":"Cervoni F","year":"1993","journal-title":"J Immunol"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.3.859"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(199902)52:2<196::AID-MRD11>3.0.CO;2-A"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(94)90447-2"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080270214"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830230630"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1998.00455.x"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"4946","DOI":"10.4049\/jimmunol.157.11.4946","article-title":"Molecular cloning of guinea pig membrane cofactor protein","volume":"157","author":"Hosokawa M","year":"1996","journal-title":"J Immunol"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(92)90376-4"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.25.15148"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1998.tb00360.x"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(93)90038-D"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5272.228"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2001.01458.x"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod64.1.36"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0378(97)01017-6"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-77014-2_4"},{"key":"e_1_2_1_30_1","doi-asserted-by":"crossref","first-page":"4415","DOI":"10.4049\/jimmunol.156.11.4415","article-title":"Membrane cofactor protein (MCP; 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