{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T16:12:17Z","timestamp":1771949537374,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,5,21]],"date-time":"2002-05-21T00:00:00Z","timestamp":1021939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Hungarian research Grant","award":["OTKA TO33126"],"award-info":[{"award-number":["OTKA TO33126"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The adrenergic system plays a major role in the regulation of the uterine contractility during pregnancy. Our previous studies have shown the significance of the \u03b1<jats:sub>1<\/jats:sub>\u2010adrenergic receptors (ARs) in the control of pregnant uterine contractility both in vitro and in vivo. Our present aim was to determine the changes in mRNA expression and pharmacological reactivity of the \u03b1<jats:sub>1<\/jats:sub>\u2010ARs on days 18, 20, and 22 of pregnancy. To demonstrate the expressions of \u03b1<jats:sub>1<\/jats:sub>\u2010AR subtype mRNA, we used a reverse transcription\u2010polymerase chain reaction (RT\u2010PCR); the pharmacological reactivity was tested by electric field stimulation (EFS). The expression of \u03b1<jats:sub>1A<\/jats:sub>\u2010AR mRNA increased from day 18 to 22, while no \u03b1<jats:sub>1B<\/jats:sub>\u2010AR mRNA was detectable. We found a small increase in the expression of \u03b1<jats:sub>1D<\/jats:sub>\u2010AR mRNA on day 20, which was not followed by a significant change in pharmacological reactivity. The \u03b1<jats:sub>1D<\/jats:sub>\u2010receptor expression and pharmacological reactivity decreased significantly up to day 22. EFS studies revealed that the \u03b1<jats:sub>1A<\/jats:sub>\u2010AR antagonist 5\u2010methylurapidil had EC50 values (1.9\u2009\u00d7\u200910<jats:sup>\u22126<\/jats:sup>\u20136.3\u2009\u00d7\u200910<jats:sup>\u22126<\/jats:sup> M) about one order of magnitude lower than those of the \u03b1<jats:sub>1D<\/jats:sub>\u2010AR antagonist BMY 7378 (4\u2009\u00d7\u200910<jats:sup>\u22126<\/jats:sup>\u20133.6\u2009\u00d7\u200910<jats:sup>\u22125<\/jats:sup> M). However, the \u03b1<jats:sub>1B<\/jats:sub>\u2010AR antagonist cyclazosine exerted only a slight effect on the stimulated contractions. Strong correlations were found between the \u03b1<jats:sub>1A<\/jats:sub>\u2010mRNA expression and the EC50 of 5\u2010methylurapidil (r<jats:sup>2<\/jats:sup>\u2009=0.9712), and between the \u03b1<jats:sub>1D<\/jats:sub>\u2010AR mRNA expression and the EC50 of BMY 7378 (r<jats:sup>2<\/jats:sup>\u2009=\u20090.9937). Our findings suggest that both \u03b1<jats:sub>1A<\/jats:sub>\u2010 and \u03b1<jats:sub>1D<\/jats:sub>\u2010ARs are involved in the regulation of the pregnant uterine contractility. The density and pharmacological reactivity indicate that the \u03b1<jats:sub>1A<\/jats:sub>\u2010AR seems to play the major role in late\u2010pregnant myometrial contraction. Mol. Reprod. Dev. 62: 343\u2013347, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.10148","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:48:25Z","timestamp":1030319305000},"page":"343-347","source":"Crossref","is-referenced-by-count":17,"title":["Altered levels of mRNA expression and pharmacological reactivity of \u03b1<sub>1<\/sub>\u2010adrenergic receptor subtypes in the late\u2010pregnant rat myometrium"],"prefix":"10.1002","volume":"62","author":[{"given":"Eszter","family":"Ducza","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00f3bert","family":"G\u00e1sp\u00e1r","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Falkay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,5,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(79)90433-3"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199207303270503"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1124\/mol.59.5.1235","article-title":"Use of antisense oligonucleotides to verify the role of the alpha (1A) andrenergic receptor in the contractility of the rat uterus post partum","volume":"59","author":"Ducza E","year":"2001","journal-title":"Mol Pharmacol"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.82.4.1235"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(98)00035-6"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(00)01014-6"},{"key":"e_1_2_1_9_1","first-page":"267","article-title":"International Union of Pharmacology X. 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