{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:12:20Z","timestamp":1761707540973},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,10,30]],"date-time":"2002-10-30T00:00:00Z","timestamp":1035936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Epididymis provides a safe environment in which stored\u2010spermatozoa could survive for days before ejaculation. In vitro studies suggested that epididymal proteins seem to be implicated in sperm survival during coincubation with cultured epididymal cells. This study was basically designed to confirm if secretory proteins from bovine epididymal cell cultures provide sperm protection against rapid loss of sperm motility in vitro. Bovine spermatozoa were incubated in conditioned media (CM), which were prepared from cultured cauda epididymal cell (CEC). Motion parameters were recorded using a computer\u2010assisted sperm analyzer. Sperm\u2010free protein extracts from CM were fractionated by ultrafiltration through a 10\u2010kDa cut off membrane. A significantly positive effect on sperm motility was observed when spermatozoa were incubated in CM (54\u2009\u00b1\u20094%) and CM\u2009&gt;10 kDa (57\u2009\u00b1\u20094%) compared to CM\u2009&lt;10\u2010kDa fraction (30\u2009\u00b1\u20093%) or fresh media (34\u2009\u00b1\u20093%), after a 6\u2010hr incubation period. This beneficial effect on sperm motility was abolished when the CM\u2009&gt;10\u2010kDa fraction was heat\u2010treated at 100\u00b0C for 10 min. The CM\u2009&gt;10 kDa fraction provides factors that remained active even though spermatozoa were washed twice after a 2\u2010hr preincubation period. To identify potential beneficial factors, bovine spermatozoa were incubated with radiolabeled proteins obtained using <jats:sup>35<\/jats:sup>S\u2010methionine in culture medium. SDS\u2013PAGE analysis of proteins extracted from CM\u2010preincubated spermatozoa revealed the presence of a 42\u2010kDa protein strongly associated to the sperm surface. This 42\u2010kDa spot was trypsin\u2010digested and identified by matrix\u2010assisted laser desorption ionization\u2010time of flight (MALDI\u2010TOF) as a protein homologue to a 35\u2010kDa bovine estrogen\u2010sulfotransferase. This protein can play a role in epididymal biology and sperm function. Taken together, these results suggest that specific epididymal proteins can be implicated in the sperm protection in vitro, and can be characterized in our cell culture system. Mol. Reprod. Dev. 63: 500\u2013509, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.10192","type":"journal-article","created":{"date-parts":[[2002,11,4]],"date-time":"2002-11-04T13:55:39Z","timestamp":1036418139000},"page":"500-509","source":"Crossref","is-referenced-by-count":19,"title":["Characterization of secretory proteins from cultured cauda epididymal cells that significantly sustain bovine sperm motility in 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