{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:06:13Z","timestamp":1792782373972,"version":"4.5.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,11,4]],"date-time":"2002-11-04T00:00:00Z","timestamp":1036368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ernst-Schering-Research foundation"},{"DOI":"10.13039\/100000877","name":"Rockefeller foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000877","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content>H<\/jats:styled-content>\n                    uman\n                    <jats:styled-content>E<\/jats:styled-content>\n                    pididymis\u2010specific protein 6 [HE6 (GPR64)] is a highly conserved, tissue\u2010specific seven\u2010transmembrane receptor of the human epididymis. The rodent counterparts were cloned and 5\u2032\u2010inverse PCR employed to confirm that the cDNA sequences were full length. Downstream from the highly conserved signal peptide\u2010coding sequence, the 5\u2032\u2010regions contained at least six mini\u2010exons of less than 50 nucleotides. Multiple splice variants involving these mini\u2010exons were cloned in the human, the majority of which was also found in rodents. Northern blot analysis showed that the tissue distribution of the mRNA was very similar in human and rodents. The human HE6 gene was assigned to the X chromosome in a region, which is syntenic to the mouse. The HE6 sequence predicted a two\u2010subunit receptor of the LNB\u2010TM7 subfamily. A membrane preparation and protein solubilization method was adopted to identify the endogenous epididymal proteins. Two sets of peptides were chosen for antibody production, assuming that protein scission had occurred within the conserved GPS\u2010motif. Western blot analysis revealed abundant two\u2010subunit proteins in human and rodents, comprising an approximately 180 kDa hydrophilic ectosubunit and a &lt;40 kDa hydrophobic endosubunit. Deglycosylation experiments showed that the large ectosubunits were highly glycosylated, the carbohydrate side chains dramatically increasing the apparent molecular mass. Immunohistochemical studies revealed that both subunits were associated with apical membranes of efferent ductule and proximal epididymal duct epithelia. Mol. Reprod. Dev. 64: 13\u201326, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/mrd.10220","type":"journal-article","created":{"date-parts":[[2002,11,6]],"date-time":"2002-11-06T14:01:46Z","timestamp":1036591306000},"page":"13-26","source":"Crossref","is-referenced-by-count":79,"title":["HE6, a two\u2010subunit heptahelical receptor associated with apical membranes of efferent and epididymal duct epithelia"],"prefix":"10.1002","volume":"64","author":[{"given":"Heike","family":"Obermann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annemarie","family":"Samalecos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Osterhoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Schr\u00f6der","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raoul","family":"Heller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane","family":"Kirchhoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,11,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.28.19957"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110877200"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.139.6.2960"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(01)01804-7"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/283541a0"},{"issue":"3","key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"526","DOI":"10.2144\/96213pf02","article-title":"Full\u2010length cDNA cloning and determination of mRNA 5\u2032 and 3\u2032 ends by amplification of adaptor\u2010ligated cDNA","volume":"21","author":"Chenchik A","year":"1996","journal-title":"Biotechniques"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00266413"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1210435"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(83)90418-9"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1160391"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/978-1-4757-2065-5","volume-title":"Biomembranes, molecular structure and function","author":"Gennis RB","year":"1989"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000234200"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.157.12.5438"},{"key":"e_1_2_1_15_1","volume-title":"Morphologische Studien am Nebenhoden des Menschen","author":"Holstein AF","year":"1969"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.6.3185"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.1990.tb00972.x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1998.tb01164.x"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0215(20000815)87:4<480::AID-IJC4>3.0.CO;2-6"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80332-3"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.6.3590"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.34.21504"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00005-8"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.13.2850"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(99)00324-1"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M004814200"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.1997.16.379"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(99)80379-0"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200105)22:9<1810::AID-ELPS1810>3.0.CO;2-M"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(00)01583-8"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.49.32715"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.9.3219"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001910306"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrd.10220","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.10220","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T14:13:43Z","timestamp":1700144023000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.10220"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,11,4]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,1]]}},"alternative-id":["10.1002\/mrd.10220"],"URL":"https:\/\/doi.org\/10.1002\/mrd.10220","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"value":"1040-452X","type":"print"},{"value":"1098-2795","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,11,4]]}}}