{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T16:56:51Z","timestamp":1764349011189},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T00:00:00Z","timestamp":1050364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>By RT\u2010PCR two transcripts for arylalkylamine <jats:italic>N<\/jats:italic>\u2010acetyltransferase (AA\u2010NAT; serotonin <jats:italic>N<\/jats:italic>\u2010acetyltransferase; EC 2.3.1.87), the key enzyme in melatonin synthesis, were found, for the first time, in the oocytes and blastoderms from freshly laid eggs (323\u2010 and 238\u2010bp RT\u2010PCR products), and one (238\u2010bp product) in the pineal gland of Japanese quail (<jats:italic>Coturnix coturnix japonica<\/jats:italic>). The two products differed by an intron of 85\u2010bp present in the 323\u2010bp band and absent from the 238\u2010bp band. The identity of the products was confirmed by restriction analysis and sequencing. The ratio of the 323:238\u2010bp bands changed during oogenesis from \u223c17:1 in small 3\u2010mm oocytes to \u223c4:1 in immature vitellogenic oocytes and \u223c1:1 in mature, preovulatory oocytes; it was reversed to \u223c0.2:1 in blastoderms from fertile freshly laid eggs, corresponding to embryo of \u223c40,000 cells. It is proposed that the longer 323\u2010bp product, containing an intron, represents a translationally inactive form of the transcript, stored in maternal RNA. The shorter 238\u2010bp product lacking an intron may represent the mature active AA\u2010NAT mRNA found in the pineal gland and in early embryos, and\u2014to a lower proportion\u2014in older oocytes. These data constitute the first direct proof of an intron sequence in maternal RNA of avian oocyte. It is possible that differential processing of the immature mRNA is part of a transcriptional regulation mechanism of AA\u2010NAT activity. A possible role of extrapineal melatonin in early avian development is discussed. Mol. Reprod. Dev. 65: 132\u2013140, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.10236","type":"journal-article","created":{"date-parts":[[2003,4,16]],"date-time":"2003-04-16T10:45:32Z","timestamp":1050489932000},"page":"132-140","source":"Crossref","is-referenced-by-count":12,"title":["Presence and developmental regulation of serotonin <i>N<\/i>\u2010acetyltransferase transcripts in oocytes and early quail embryos (<i>Coturnix coturnix 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