{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T15:16:41Z","timestamp":1771687001796,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,12,24]],"date-time":"2002-12-24T00:00:00Z","timestamp":1040688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Defective sperm volume regulation causes infertility in c\u2010<jats:italic>ros<\/jats:italic>knockout (KO) mice lacking the initial segment of the epididymis (IS). As taurine is in high concentration in the male tract and acts as an organic osmolyte in somatic cells, the taurine transporter (TauT), taurine content and taurine channel phospholemman (PLM) were examined in the epididymides of these and fertile heterozygous (+\/\u2212) mice. TauT and PLM genes were demonstrated by RT\u2010PCR in the caput of +\/\u2212 and c\u2010<jats:italic>ros<\/jats:italic>knockout males. Northern blots revealed one transcript of<jats:italic>TauT<\/jats:italic>(8 kb) in all regions with different expression between segments (cauda\u2009&gt;\u2009corpus\u2009&gt;\u2009IS\u2009&gt;\u2009caput) and no differences in expression between genotypes. Western blotting revealed three translation products of TauT in all epididymal regions (107, 69, 49 kDa) with higher expression of the 69 kDa and 49 isoforms in the \u2212\/\u2212 than +\/\u2212 caput. Immunohistochemical staining revealed staining of principal cells and stronger staining of apical and clear cells in all epididymal regions. The expression of the PLM transcript (0.75 kb: cauda\u2009=\u2009corpus\u2009&gt; caput\u2009&gt;\u2009IS) was upregulated in the proximal caput and cauda of KO mice. Tissue taurine was higher in the cauda &gt;corpus&gt;IS\u2009\u2245\u2009caput in fertile males and significantly higher in the proximal caput of the KO male. By contrast, taurine concentrations in cauda epididymidal fluid and content per 10<jats:sup>6<\/jats:sup>sperm did not differ between genotypes. TauT and PLM may be involved in taurine regulation in the normal epididymis and the proximal accumulation of taurine in the infertile males. Mol. Reprod. Dev. 64: 144\u2013151, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.10250","type":"journal-article","created":{"date-parts":[[2002,12,31]],"date-time":"2002-12-31T01:03:43Z","timestamp":1041296623000},"page":"144-151","source":"Crossref","is-referenced-by-count":24,"title":["Expression and location of taurine transporters and channels in the epididymis of infertile c\u2010<i>ros<\/i>receptor tyrosine kinase\u2010deficient and fertile heterozygous mice"],"prefix":"10.1002","volume":"64","author":[{"given":"Y.\u2010X.","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Wagenfeld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.\u2010H.","family":"Yeung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Lehnert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.G.","family":"Cooper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,12,24]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod29.3.548"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1140225"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1990.tb01581.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(01)00497-8"},{"key":"e_1_2_1_6_1","first-page":"425","article-title":"Osmoregulatory alterations in taurine uptake by cultured human and bovine lens epithelial cells","volume":"43","author":"Cammarata PR","year":"2002","journal-title":"Invest Ophthal Vis Sci"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.82.3.367"},{"key":"e_1_2_1_8_1","first-page":"1","volume-title":"Encyclopedia of reproduction","author":"Cooper TG","year":"1998"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0770271"},{"key":"e_1_2_1_10_1","first-page":"R246","article-title":"Taurine, betaine, and inositol share a volume\u2010sensitive transporter in skate erythrocyte cell membrane","volume":"67","author":"Goldstein L","year":"1994","journal-title":"Am J Physiol"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.t01-1-00147.x"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.1681\/ASN.V1091874","article-title":"Ser\u2010322 is a critical site for PKC regulation of the MDCK cell taurine transporter (pNCT)","volume":"10","author":"Han X","year":"1999","journal-title":"J Am Soc Nephrol"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1990.tb00186.x"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)80008-I"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.24.12145"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215540004800301"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-169X.1980.00483.x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-0117-0_28"},{"key":"e_1_2_1_19_1","first-page":"252","article-title":"Volume sensitive efflux of taurine in HEK293 cells overexpressing phospholemman","volume":"496","author":"Morales\u2010Mulia M","year":"2002","journal-title":"Biochim Biophys Acta"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(01)00082-9"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402100113"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000016369"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(00)00315-1"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3000893"},{"key":"e_1_2_1_25_1","first-page":"999","volume-title":"The physiology of reproduction","author":"Robaire B","year":"1994"},{"key":"e_1_2_1_26_1","first-page":"563","article-title":"Cloning and expression of a high affinity taurine transporter from rat brain","volume":"42","author":"Smith KE","year":"1992","journal-title":"Mol Pharmacol"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(97)01440-4"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb04937.x"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.10.10.1184"},{"key":"e_1_2_1_30_1","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1021\/ac60139a006","article-title":"Automatic recording apparatus for use in the chromatography of amino acids","volume":"30","author":"Spackman DH","year":"1958","journal-title":"Anal Chem"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.17.8230"},{"key":"e_1_2_1_32_1","doi-asserted-by":"crossref","DOI":"10.1002\/j.1939-4640.2002.tb02333.x","article-title":"Lack of glutamate transporter EAAC1 in the epididymis of infertile c\u2010ros receptor tyrosine\u2010kinase deficient mice","volume":"23","author":"Wagenfeld A","year":"2002","journal-title":"J Androl"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(97)80470-9"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00218369"},{"key":"e_1_2_1_35_1","first-page":"137","article-title":"Receptor tyrosine kinase c\u2010ros knockout mice as a model for the study of epididymal regulation of sperm function","volume":"53","author":"Yeung CH","year":"1998","journal-title":"J Reprod Fertil"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.4.1062"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod63.2.612"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2002.tb02274.x"},{"key":"e_1_2_1_39_1","first-page":"F646","article-title":"Anion dependence of taurine transport by rat renal brush\u2010border membrane vesicles","volume":"256","author":"Zelikovic I","year":"1989","journal-title":"Am J Physiol"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.10250","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T17:44:51Z","timestamp":1704735891000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.10250"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,12,24]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,2]]}},"alternative-id":["10.1002\/mrd.10250"],"URL":"https:\/\/doi.org\/10.1002\/mrd.10250","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"value":"1040-452X","type":"print"},{"value":"1098-2795","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,12,24]]}}}