{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T05:13:24Z","timestamp":1761196404509},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,5,29]],"date-time":"2003-05-29T00:00:00Z","timestamp":1054166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bovine oocytes are arrested at the prophase of first meiotic cell cycle. Meiosis resumes in oocytes of pre\u2010ovulatory follicles upon LH surge. However, oocytes from secondary follicles spontaneously resume meiosis in the absence of hormones if removed from the follicle and cultured in vitro. The nature of meiotic arrestor in bovine follicles is poorly understood. In this study we investigated the role of cell\u2013cell interactions between granulosa and cumulus cells and the oocyte in mediating maintenance of meiotic arrest by cAMP. We sorted oocytes as granulosa\u2013cumulus oocyte complexes (GCOC) if surrounded with cumulus cells attached to a large granulosa investment or cumulus oocytes complexes (COC) if surrounded with cumulus cells only and investigated the role cAMP in maintenance of meiotic arrest in these oocytes under various conditions. In hormone\u2010 and serum\u2010free medium both GCOC and COC enclosed oocytes resumed meiosis. When [cAMP]<jats:sub>i<\/jats:sub> was elevated with addition of invasive adenylate cyclase (iAC) GCOC enclosed oocytes were maintained in the prophase with intact germinal vesicle (GV) while COC enclosed oocytes underwent GV breakdown (GVBD). iAC elevated [cAMP]<jats:sub>i<\/jats:sub> in both types of oocytes to the same level. If oocytes were liberated from the cumulus and granulosa cells, they re\u2010initiated meiosis in serum and hormone free medium, but remained in the GV stage if iAC was added to the medium. Untreated GCOC and COC enclosed oocytes extruded first polar body at the same frequency in hormone\u2010supplemented media. GCOC and COC enclosed oocytes but not denuded oocytes (DO) cultured without somatic cells acquired developmental competence if cultured in hormone\u2010containing medium. It is concluded that maintenance of meiotic arrest is regulated by the interplay of [cAMP]<jats:sub>i<\/jats:sub>, and cumulus and granulosa cells. Mol. Reprod. Dev. 65: 336\u2013343, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.10287","type":"journal-article","created":{"date-parts":[[2003,5,30]],"date-time":"2003-05-30T10:06:10Z","timestamp":1054289170000},"page":"336-343","source":"Crossref","is-referenced-by-count":8,"title":["Meiotic state of bovine oocytes is regulated by interactions between cAMP, cumulus, and granulosa"],"prefix":"10.1002","volume":"65","author":[{"given":"Huseyin","family":"Aktas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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