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Results show that progressive removal of seminal plasma from ram spermatozoa by repeated centrifugation and resuspension in media\u2009\u00b1\u20094% bovine serum albumin (BSA) or 0.4% polyvinlypyrrolidone (PVP) causes a reduction in lipid diffusion in all regions of the membrane. By contrast, bull sperm membranes respond with an increase in diffusion in all regions. Repeated washing of bull spermatozoa whose membranes were previously immobile (i.e., showed no recovery after FRAP) restored lipid diffusion suggesting an inhibitory effect of seminal plasma proteins. Further analysis by atomic force microscopy revealed a close association between BSA and the plasma membrane. It is concluded that diffusion of lipids in the plasma membrane of ejaculated ram and bull spermatozoa is influenced by seminal plasma proteins and the composition of the suspending medium. <jats:italic>Mol. Reprod. Dev.<\/jats:italic> 59:306\u2013313, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.1035","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:43:33Z","timestamp":1030311813000},"page":"306-313","source":"Crossref","is-referenced-by-count":5,"title":["Lipid dynamics in the plasma membrane of ram and bull spermatozoa after washing and exposure to macromolecules BSA and PVP"],"prefix":"10.1002","volume":"59","author":[{"given":"Caroline A.","family":"Wolfe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter S.","family":"James","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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