{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:02:50Z","timestamp":1760644970332},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4601,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[1992,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have established a monolayer culture system for human fallopian tube epithelial cells. The cells were isolated from tubes using collagenase digestion, and were cultured in Ham's F\u201010 supplemented with 15% fetal calf serum. The epithelial cells derived from culture were characterized using immunocytochemical staining and electron microscopy. These cells were stained with antikeratin and anti\u2010epithelial membrane antigen, but showed no staining after treatment with antivimentin. Electron microscopy showed many microvilli on the cell surface and tight junctions or desmosomes at areas of cell\u2010cell contact. Cell proliferation was enhanced by epidermal growth factor, but not by fibroblast growth factor, insulin, transferrin, estradiol\u201017\u03b2, or progesterone.<\/jats:p><jats:p>The 2\u2010cell ICR mouse pre\u2010embryos were co\u2010cultured for 4 days with tubal epithelial cells (A) (n = 98), in cell\u2010conditioned medium (B) (n = 83), or in medium alone (C) (n = 72). During the first 24 h in culture, for groups A and B, the rates of cleavage to the 4\u2010cell stage were 90.9% and 81.9%, respectively. Cleavage rates in these two groups were significantly higher (<jats:italic>P<\/jats:italic> =0.0012, <jats:italic>P<\/jats:italic> &gt; 0.00001) than in group C (56.9%). After 72 h in culture, the rates of development to the blastocyst stage were significantly higher for groups A and B compared to group C (89.6% and 73.5% vs. 54.5%, <jats:italic>P<\/jats:italic> &gt; 0.00001, <jats:italic>P<\/jats:italic> = 0.0002). These results suggest that factor(s) from tubal epithelial cells may facilitate the development of mouse pre\u2010embryos throughout the pre\u2010implantation stages.<\/jats:p>","DOI":"10.1002\/mrd.1080320308","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T00:09:39Z","timestamp":1109203779000},"page":"236-242","source":"Crossref","is-referenced-by-count":12,"title":["Primary culture of human fallopian tube epithelial cells and co\u2010culture of early mouse pre\u2010embryos"],"prefix":"10.1002","volume":"32","author":[{"given":"Kazuhiro","family":"Takeuchi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukihiro","family":"Nagata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce A.","family":"Sandow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary D.","family":"Hodgen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod24.4.852"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod43.3.457"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod43.2.340"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02624213"},{"key":"e_1_2_1_6_1","volume-title":"Biostatistics: A Foundation for Analysis in the Health Sciences","author":"Daniels WW","year":"1987"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0890293"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0850715"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0810023"},{"key":"e_1_2_1_10_1","first-page":"246A","article-title":"Estrus\u2010associated 98 kDa protein in bovine oviductal fluid (ODF)","volume":"38","author":"Gerena RL","year":"1988","journal-title":"Biol Reprod"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1071\/BI9820187"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(87)90070-7"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(85)90122-8"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod42.2.301"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1120190202"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod31.1.165"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(88)90242-7"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod38.3.687"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0900109"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0770645"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02633217"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-124-5-2464"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-122-4-1639"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod41.3.425"},{"key":"e_1_2_1_25_1","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/S0015-0282(16)60926-3","article-title":"Coculture of human zygotes on fetal bovine uterine fibroblasts:embryonic morphology and implantation","volume":"52","author":"Wiemer KE","year":"1989","journal-title":"Fertil Steril"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrd.1080320308","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.1080320308","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T10:36:58Z","timestamp":1698057418000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.1080320308"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,7]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1992,7]]}},"alternative-id":["10.1002\/mrd.1080320308"],"URL":"https:\/\/doi.org\/10.1002\/mrd.1080320308","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"value":"1040-452X","type":"print"},{"value":"1098-2795","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,7]]}}}