{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:37:22Z","timestamp":1761662242452},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4236,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[1993,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tissue inhibitors of metalloproteinases (TIMPs) appear to play an important regulatory role in tissue remodelling and invasion by malignant cells. Since pregnancy involves morphological changes in existing maternal tissues, as well as a strictly controlled invasion by fetal trophoblasts, we have examined the temporal expression of TIMP\u20101, TIMP\u20102, and specific metalloproteinases in the mouse uterus, decidua, placenta, amnion, and ovaries throughout gestation by examining mRNA levels on northern and slot blots. Maximal levels of TIMP\u20101 mRNA were observed from day 6 to day 10 in the uterus, decidua, and placenta. In clear contrast to the early burst of TIMP\u20101 mRNA accumulation, the level of TIMP\u20102 mRNA increased steadily throughout gestation in the uterus, decidua, and amnion, while in the placenta it showed a sevenfold increase after day 14. In amnion, TIMP\u20101 was induced specifically on day 18. Interestingly, the normally high level of TIMP\u20101 mRNA seen in the ovaries of virgin mice was low during gestation, until day 18 and postpartum, when a sixfold increase over the levels in virgin ovary was observed. In contrast, ovarian TIMP\u20102 mRNA showed a marginal increase during gestation. The temporal pattern of 72 kDa gelatinase type A followed that of TIMP\u20102 in the decidua and ovary. Stromelysin\u20102 mRNA was detected at term only in ovary and decidua. Our data show that the temporal accumulation of TIMP\u20101 and TIMP\u20102 mRNA is precisely coordinated in each of the tissue compartments and is independently regulated during the in vivo remodelling of reproductive tissues in gestation. The peak of TIMP\u20101 mRNA levels in the uterus, decidua, and placenta at midgestation is associated with the most invasive period of embryo development. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.1080350302","type":"journal-article","created":{"date-parts":[[2005,2,23]],"date-time":"2005-02-23T23:53:50Z","timestamp":1109202830000},"page":"219-226","source":"Crossref","is-referenced-by-count":49,"title":["Temporal expression of tissue inhibitors of metalloproteinases in mouse reproductive tissues during gestation"],"prefix":"10.1002","volume":"35","author":[{"given":"Paul","family":"Waterhouse","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David 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