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In the present study we investigated the possible presence of IFN\u03b3 receptors (IFNGR) on mouse oocytes and preimplantation embryos. The two receptor chains IFN\u03b3R\u03b1 (IFNGR\u20101) and IFN\u03b3R\u03b2 (IFNGR\u20102) have been detected by indirect immunofluorescence at the surface of mouse oocytes (in germinal vesicle and metaphase II stages), as well as at all stages of in vitro embryo development from the one\u2010cell to blastocyst stage. IFNGR appeared to colocalize partly with ganglioside GM1 at the cell surface of oocytes and embryos, indicating a possible preferential localization of this receptor in \u201crafts\u201d microdomains. This was analyzed in more detail using software developed in the laboratory. IFN\u03b3 was found to bind to its receptor at all stages analyzed. RT\u2010PCR and Southern blot experiments confirmed the presence of the transcriptionally regulated IFNGR\u20102 chain mRNA, in mouse oocytes and preimplantation embryos. These results show, for the first time, that mouse oocytes and preimplantation embryos bear a complete and theoretically functional IFNGR, suggesting that this cytokine could play a role during early development.Mol. Reprod. Dev. 60: 319\u2013330, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.1094","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T20:44:49Z","timestamp":1030308289000},"page":"319-330","source":"Crossref","is-referenced-by-count":24,"title":["Mouse oocytes and preimplantation embryos bear the two sub\u2010units of interferon\u2010gamma receptor"],"prefix":"10.1002","volume":"60","author":[{"given":"Sandrine","family":"Truchet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juana","family":"Wietzerbin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale","family":"Debey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,9,28]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.111.23.3551"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1988.tb00197.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.2.493"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.15.1.563"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9668"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1995.1130"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.14.1.111"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/14.4.959"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0890447"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(99)00306-4"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1999.tb00071.x"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8456300"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1020185"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/14.12.3060"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080360110"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.136.11.4925"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.26.15623"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.11.040193.003035"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.21.8497"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1997.1199"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.141.4.929"},{"key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1242\/dev.121.4.1005","article-title":"Proteinase 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