{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T17:48:22Z","timestamp":1771609702704,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,17]],"date-time":"2005-02-17T00:00:00Z","timestamp":1108598400000},"content-version":"vor","delay-in-days":5771,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gamete Research"],"published-print":{"date-parts":[[1989,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Porcine morulae were bisected by a glass needle after softening of zonae pcllucidae by pronase followed by a treatment with 0.05% trypsin\/0,02% EDTA for decreasing intercel lular junction of blastomeres. Transfer of 49 half\u2010morulae to three recipients resulted in one pregnancy. The blastocysts were bisected symmetrically so as to leave a cellular bridge between the sister half\u2010embryos after the softening of zonae followed by or without the trypsin\/EDTA treatment. Transfer of 47 monozygotic (MZ) pairs of half\u2010blastocysts to nine recipients resulted in four pregnancies. A litter of nine fetuses was obtained from seven MZ pairs of half\u2010blastocysts, demonstrating that at least two pairs of MZ twin fetuses were produced. It is thought that the procedure for bisecting blastocysts developed in this study is one of the potential methods of producing porcine MZ twins.<\/jats:p>","DOI":"10.1002\/mrd.1120230102","type":"journal-article","created":{"date-parts":[[2005,2,25]],"date-time":"2005-02-25T12:36:02Z","timestamp":1109334962000},"page":"1-9","source":"Crossref","is-referenced-by-count":23,"title":["Microsurgical bisection of porcine morulae and blastocysts to produce monozygotic twin pregnancy"],"prefix":"10.1002","volume":"23","author":[{"given":"Hiroshi","family":"Nagashima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukio","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shyoso","family":"Ogawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0710607"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(88)90042-8"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(86)90108-1"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(84)90440-0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(83)90027-4"},{"key":"e_1_2_1_7_1","first-page":"242","article-title":"Experimentelle Erzuegung von identischen M\u00e4usezwillingen","volume":"85","author":"Moustafa VLA","year":"1978","journal-title":"Dtsch Tier\u00e4rztl Wochenschr"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd1977.27.12"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0700357"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(87)90139-7"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(88)90250-6"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0690463"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.110.6.126"},{"key":"e_1_2_1_14_1","first-page":"93","article-title":"How does embryo manipulation fit into present and future pig reproduction?","volume":"33","author":"Polge C","year":"1985","journal-title":"J Reprod Fertil [Suppl]"},{"key":"e_1_2_1_15_1","first-page":"101","article-title":"Manipulation of gametes and embryos in the pig","volume":"33","author":"Robl JM","year":"1985","journal-title":"J Reprod Fertil [Suppl]"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(85)90131-1"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0690315"},{"key":"e_1_2_1_18_1","first-page":"643","article-title":"Production of monozygotic twins following transfer of separated half embryos in the goat","volume":"55","author":"Tsunoda Y","year":"1984","journal-title":"Jpn J Zootechnic"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(85)90225-0"},{"key":"e_1_2_1_20_1","first-page":"7","article-title":"Culture of mouse ova","volume":"14","author":"Whittingham DG","year":"1971","journal-title":"J Reprod Fertil [Suppl]"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/277298a0"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0590357"},{"key":"e_1_2_1_23_1","first-page":"165","article-title":"The developmental capacity of blastomeres from 4\u2010 and 8\u2010cell sheep embryos","volume":"65","author":"Willadsen SM","year":"1981","journal-title":"J Embryol Exp Morphol"},{"key":"e_1_2_1_24_1","first-page":"185","volume-title":"Mammalian Egg Transfer","author":"Willadsen SM","year":"1982"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.108.10.211"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.114.10.240"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(81)80015-5"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(82)90158-3"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(84)90051-7"}],"container-title":["Gamete Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrd.1120230102","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.1120230102","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T03:39:19Z","timestamp":1697945959000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.1120230102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,5]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,5]]}},"alternative-id":["10.1002\/mrd.1120230102"],"URL":"https:\/\/doi.org\/10.1002\/mrd.1120230102","archive":["Portico"],"relation":{},"ISSN":["0148-7280","1554-3919"],"issn-type":[{"value":"0148-7280","type":"print"},{"value":"1554-3919","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,5]]}}}