{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:59:01Z","timestamp":1767855541351,"version":"3.49.0"},"reference-count":60,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,11,21]],"date-time":"2003-11-21T00:00:00Z","timestamp":1069372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Genetic understanding of male\u2010factor infertility requires knowledge of gene expression patterns associated with normal germ cell differentiation. The mouse is one of the best models of mammalian fertility due to its well\u2010characterized genetics and the existence of many infertile mutants both naturally occurring and experimentally induced. We used cDNA microarrays firstly to investigate normal gene expression in the wild\u2010type (wt) testis and secondly to gain a better insight into the effect of the disruption of the <jats:italic>Dazl<\/jats:italic> gene on spermatogenesis. We constructed a cDNA microarray from a subtracted and normalized adult testis library and focused on six developmental time\u2010points during the initial synchronous wave of spermatogenesis. The results suggest that in the wild\u2010type testis, 89.5% of genes on our chip change expression dramatically during the time\u2010course. To identify patterns in the gene\u2010expression data, a <jats:italic>k<\/jats:italic>\u2010means clustering algorithm and principal component analysis were used. In the <jats:italic>Dazl<\/jats:italic> knockout testes, the majority of genes remain at baseline levels of expression, because absence of <jats:italic>Dazl<\/jats:italic> has a severe effect on cell\u2010types present in the testis. Although in the prepubescent <jats:italic>Dazl<\/jats:italic>\u2010null mice the final point reached in germ cell development is the leptotene\u2010zygotene stage, the microarray results suggest that lack of <jats:italic>Dazl<\/jats:italic> expression has a detectable effect on the mRNA complement of germ cells as early as day 5 when only type A spermatogonia are present. Mol. Reprod. Dev. 67: 26\u201354, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20010","type":"journal-article","created":{"date-parts":[[2003,12,2]],"date-time":"2003-12-02T14:17:23Z","timestamp":1070374643000},"page":"26-54","source":"Crossref","is-referenced-by-count":39,"title":["Expression profiling of the developing testis in wild\u2010type and <i>Dazl<\/i> knockout mice"],"prefix":"10.1002","volume":"67","author":[{"given":"Klio","family":"Maratou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thorsten","family":"Forster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yael","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary","family":"Taggart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert M.","family":"Speed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Ireland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Teague","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas","family":"Roy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Howard J.","family":"Cooke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,11,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.18.10101"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.10.12.2030"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03343782"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/7.1.102"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.74.1.68"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.9.2.659"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(199604)43:4<403::AID-MRD1>3.0.CO;2-T"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/mr.60.1.233-249.1996"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/5.4.513"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.21.3452"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.84.10.3443"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1210347"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.22.e33"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.11.5717"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.1.398"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kvf073"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1242\/jcs.65.1.249","article-title":"Morphological and temporal sequence of meiotic prophase development at puberty in the male mouse","volume":"65","author":"Goetz P","year":"1984","journal-title":"J Cell Sci"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1053\/beem.2000.0083"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-1878(199807)20:7<555::AID-BIES6>3.0.CO;2-J"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1030"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.150242097"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.2.475"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00354930"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.309.6954.570"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(96)66011-X"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2443.2002.00576.x"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/7.4.715"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/11091"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod59.1.84"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1999.tb02565.x"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2002-0059"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0234478100"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.134.3482.832"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)09899-9"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0895-383"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1996.0366"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)00059-9"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(00)00219-5"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/37987"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1260589"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod65.3.771"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/8.6.511"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004290100176"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.14.7.841"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0671"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/10343"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2000.6169"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.6.1655"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-437X(99)80052-5"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.12.2479"},{"key":"e_1_2_1_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00701-6"},{"key":"e_1_2_1_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/86927"},{"key":"e_1_2_1_55_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1995.1169"},{"key":"e_1_2_1_56_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.131090498"},{"key":"e_1_2_1_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg017"},{"key":"e_1_2_1_58_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.6.1694"},{"key":"e_1_2_1_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00005-2"},{"key":"e_1_2_1_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80404-9"},{"key":"e_1_2_1_61_1","doi-asserted-by":"crossref","first-page":"2318","DOI":"10.1210\/endo.142.6.8106","article-title":"Male germ cells regulate transcription of the cathepsin l gene by rat Sertoli cells","volume":"142","author":"Zabludoff SD","year":"2001","journal-title":"Endocrinology"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrd.20010","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.20010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T13:44:17Z","timestamp":1700228657000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.20010"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,11,21]]},"references-count":60,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1002\/mrd.20010"],"URL":"https:\/\/doi.org\/10.1002\/mrd.20010","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"value":"1040-452X","type":"print"},{"value":"1098-2795","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,11,21]]}}}