{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T22:56:43Z","timestamp":1793055403610,"version":"4.5.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,11,21]],"date-time":"2003-11-21T00:00:00Z","timestamp":1069372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>River buffalo, sheep, and goat spermatozoa were cross\u2010hybridized using double color fluorescence in situ hybridization (FISH) with bovine Xcen\u2010 and Y\u2010chromosome painting probes, prepared by DOP\u2010PCR of laser\u2010microdissected\u2010catapulted chromosomes, to investigate the possibility of using bovine probes for sexing sperm of other members of the family Bovidae. Before sperm analysis, the probes were hybridized on metaphase chromosomes of each species, as control. Frozen\u2010thawed spermatozoa of cattle, river buffalo, sheep, and goat were decondensed in suspension with 5 mM DTT. Sperm samples obtained from three individuals of each species were investigated, more than 1,000 spermatozoa were scored in each animal. FISH analysis of more than 12,000 sperm revealed high level of sperm with X\u2010 or Y\u2010signals in all of the species investigated, indicating FISH efficiency over 99%. Significant interspecific differences were detected in the frequency of aberrant spermatozoa (aneuploid and diploid) between goat (0.393%) and sheep (0.033%) (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), goat and cattle (0.096%) (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.5), as well as between river buffalo (0.224%) and sheep (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.5). There was no significant difference between river buffalo and cattle. The present study demonstrated that it is possible to use bovine X\u2010Y painting probes for sexing and analyzing sperm of other species of the family, thus facilitating future studies on the incidence of chromosome abnormalities in sperm as well as on sex predetermination of embryos for the livestock industry. Mol. Reprod. Dev. 67: 108\u2013115, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20016","type":"journal-article","created":{"date-parts":[[2003,12,2]],"date-time":"2003-12-02T14:17:23Z","timestamp":1070374643000},"page":"108-115","source":"Crossref","is-referenced-by-count":31,"title":["Sexing river buffalo (<i>Bubalus bubalis<\/i> L.), sheep (<i>Ovis aries<\/i> L.), goat (<i>Capra hircus<\/i> L.), and cattle spermatozoa by double color FISH using bovine (<i>Bos taurus<\/i> L.) X\u2010 and Y\u2010painting probes"],"prefix":"10.1002","volume":"67","author":[{"given":"Dino","family":"Di Berardino","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miluse","family":"Vozdova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svatava","family":"Kubickova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Halina","family":"Cernohorska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Coppola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianfranco","family":"Coppola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Enne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiri","family":"Rubes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,11,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000015286"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000130877"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1013185330114"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000133808"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000015148"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1139\/g81-011"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1093\/oxfordjournals.jhered.a111215","article-title":"Chromosome conservation in the Bovidae","volume":"83","author":"Gallagher DS","year":"1992","journal-title":"J Hered"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080330211"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009263718667"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(199908)53:4<407::AID-MRD6>3.0.CO;2-O"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000133114"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000133593"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000133808"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00713067"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/00087114.1990.10797007"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02259710"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000015607"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000132898"},{"key":"e_1_2_1_20_1","first-page":"283","article-title":"International System for Chromosome Nomenclature of Domestic Bovids","volume":"92","author":"ISCNDB","year":"2000","journal-title":"Cytogenet Cell Genet"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080400409"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(199604)43:4<548::AID-MRD18>3.0.CO;2-V"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00193-4"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020914702767"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1159\/000015004"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9686-33-1-89"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.8.3450"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1210541"},{"key":"e_1_2_1_29_1","unstructured":"RevayT KovacsA PresicceGA SenatoreEM NegliaG RensW GustavssonI.2002.FISH analysis of X\u2010 and Y\u2010bearing water buffalo spermatozoa. Proc 15th Eur Coll Cytogenet Dom Anim Gene Mapping June 2\u20134 Sorrento Italy (poster P33)."},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9950799"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1159\/000014976"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1159\/000015293"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080300106"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrd.20016","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.20016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T13:44:40Z","timestamp":1700228680000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.20016"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,11,21]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1002\/mrd.20016"],"URL":"https:\/\/doi.org\/10.1002\/mrd.20016","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"value":"1040-452X","type":"print"},{"value":"1098-2795","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,11,21]]}}}