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A 282 nt cDNA fragment of ovine PH\u201020 was identified in total RNA extracts of sheep testes, which exhibited 76% identity at the nucleotide level with the equivalent region of the human sequence. Ovine PH\u201020 mRNA and immunoreactivity were identified only in adult ram testis and not in peri\u2010pubertal ram testis tubules lacking round spermatids, nor in adult sheep brain, pituitary, heart, spleen, lung, liver, kidney, epididymis, or ovary. Ovine PH\u201020 protein was distributed predominantly on the postacrosomal membrane and was also present on the anterior membrane of the sperm head in fresh, unheated sheep semen. Scrotal heating caused a significant, transient decrease in the percentage of PH\u201020 immunoreactive sperm, but did not change the pattern of PH\u201020 staining on the sperm head. The results strongly suggest that ovine PH\u201020 is postmeiotically expressed in haploid germ cells in sheep testis and is arrayed on the membrane of the mature ovine spermatozoon. Scrotal heating appears to have few effects on PH\u201020 expression and distribution on ejaculated sperm. Mol. Reprod. Dev. 68: 103\u2013114, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20049","type":"journal-article","created":{"date-parts":[[2004,3,19]],"date-time":"2004-03-19T16:01:46Z","timestamp":1079712106000},"page":"103-114","source":"Crossref","is-referenced-by-count":19,"title":["Effects of scrotal heating on sperm surface protein PH\u201020 expression in sheep"],"prefix":"10.1002","volume":"68","author":[{"given":"Jean S.","family":"Fleming","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fu","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin M.","family":"McDonald","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stuart A.","family":"Meyers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grant 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