{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T10:19:15Z","timestamp":1768558755944,"version":"3.49.0"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,3,18]],"date-time":"2004-03-18T00:00:00Z","timestamp":1079568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effects of heat shock (HS) on the nucleus and cytoskeleton of in vitro matured pig oocytes were examined in this study. Porcine cumulus\u2013oocyte complexes (COCs) were aspirated from 3 to 6 mm diameter follicles and subjected to standard in vitro maturation (IVM) procedures for 42 hr. In Experiment 1, IVM\u2010derived oocytes were then randomly allocated to different HS treatments at 41.5\u00b0C for 0 (control, C0h, n\u2009=\u2009101), 1 (HS1h, n\u2009=\u2009113), 2 (HS2h, n\u2009=\u2009104), and 4 hr (HS4h, n\u2009=\u2009111), respectively. An additional control group of oocytes was cultured for 4 hr without HS (C4h, n\u2009=\u200993). Immunocytochemical staining was performed using anti\u2010tubulins and FITC\u2010conjugated mouse IgG for microtubule (MT) labeling. The chromatin and microfilaments (MFs) were stained using Hoechst 33342 and rhodamine\u2013phalloidin, respectively. In the severe HS (4 hr), the chromosomes of the MII oocytes became an aggregated chromatin structure and separated into groups. The spindle MTs were completely depolymerized or formed MT arrays. The relative fluorescence intensity (RFI) or amount of the MF structures including transzona processes (TZPs), vitelline ring (VR), and pericytoplasmic MF, were changed in various degrees. The reversibility of these alterations in the chromatin and the cytoskeleton depended on the duration of the HS. In general, abnormalities in the chromosomes, spindle MTs and the percentages of oocytes with pericytoplasmic MTs increased with length of HS treatment. The size of the spindle and the RFI of MFs in the HS oocytes were also altered. The significant changes in the nucleus and the cytoskeleton in porcine oocytes after HS may be associated with reduced development under hyperthermia and, perhaps, with the low pregnancy rates in domestic species during hot seasons. Mol. Reprod. Dev. 68: 125\u2013133, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20054","type":"journal-article","created":{"date-parts":[[2004,3,19]],"date-time":"2004-03-19T16:01:46Z","timestamp":1079712106000},"page":"125-133","source":"Crossref","is-referenced-by-count":74,"title":["Nuclear and cytoskeletal alterations of in vitro matured porcine oocytes under hyperthermia"],"prefix":"10.1002","volume":"68","author":[{"given":"Jyh\u2010Cherng","family":"Ju","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung\u2010Kai","family":"Tseng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,3,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(199812)51:4<395::AID-MRD6>3.0.CO;2-Y"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0309-1651(81)90009-6"},{"key":"e_1_2_1_4_1","first-page":"912","article-title":"In vitro culture temperatures and subsequent viability of rabbit ova","volume":"24","author":"Alliston CW","year":"1965","journal-title":"J Anim Sci"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod50.1.103"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod52.6.1296"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(85)80800-6"},{"key":"e_1_2_1_8_1","first-page":"25","article-title":"Diploid parthenogenetic mouse embryos produced by heat\u2010shock and cytochalasin B","volume":"35","author":"Balakier H","year":"1976","journal-title":"J Embryol Exp Morph"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(81)90111-7"},{"key":"e_1_2_1_10_1","first-page":"309","article-title":"Embryonic mortality caused by maternal heat stress during mouse oocyte maturation","volume":"14","author":"Baumgartner AP","year":"1987","journal-title":"Anim Prod Sci"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0840469"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1071\/BI9540552"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(54)90063-4"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(85)80985-1"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(91)90055-Z"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0248-4900(99)80082-3"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1971.3261216x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"713","DOI":"10.2527\/jas1963.223713x","article-title":"Critical period of early embryos mortality in ewes exposed to high ambient temperature","volume":"22","author":"Dutt RH","year":"1959","journal-title":"J Anim Sci"},{"key":"e_1_2_1_19_1","first-page":"123","article-title":"Temperature and light as factors in reproduction among farm animals","volume":"43","author":"Dutt RH","year":"1961","journal-title":"J Dairy Sci (Suppl)"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"86","DOI":"10.2527\/jas1971.33186x","article-title":"Early embryo development in the mammal. 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