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In vitro matured cumulus\u2013oocyte complexes (COCs) were inseminated in the presence of a variety of carbohydrates and glycoproteins to determine which glycoconjugates act as competitive inhibitors of oocyte penetration. Among the carbohydrates and glycoproteins tested, <jats:sc>D<\/jats:sc>\u2010mannose, fucoidan, dextran sulfate, and fibronectin were the most potent inhibitors of oocyte penetration (90% or more inhibition), while <jats:sc>L<\/jats:sc>\u2010fucose and vitronectin inhibited the penetration rate to a lesser extent (around 50% inhibition). Other carbohydrates caused less than 40% inhibition (i.e., <jats:sc>D<\/jats:sc>\u2010galactose, <jats:italic>N<\/jats:italic>\u2010acetyl\u2010<jats:sc>D<\/jats:sc>\u2010galactosamine, <jats:sc>D<\/jats:sc>\u2010fucose, and sialic acid) or were not effective as inhibitors of oocyte penetration (i.e., mannan, <jats:italic>N<\/jats:italic>\u2010acetyl\u2010<jats:sc>D<\/jats:sc>\u2010glucosamine, dextran, and heparan sulfate). Heparin was the only carbohydrate that significantly increased the penetration rate. To exclude a possible toxic effect on spermatozoa, sperm motility was evaluated over time by means of computer\u2010assisted sperm analysis in the presence of carbohydrates and\/or glycoproteins that inhibited the penetration rate with 40% or more. <jats:sc>L<\/jats:sc>\u2010fucose, dextran sulfate, and vitronectin did not significantly influence total and progressive sperm motility, whereas <jats:sc>D<\/jats:sc>\u2010mannose, fucoidan, and fibronectin caused a significant, but slight reduction in both motility parameters. These results are indicative for the involvement of <jats:sc>D<\/jats:sc>\u2010mannose, <jats:sc>L<\/jats:sc>\u2010fucose, fucoidan, dextran sulfate, fibronectin, and vitronectin in bovine IVF. Mol. Reprod. Dev. 68: 492\u2013499, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20095","type":"journal-article","created":{"date-parts":[[2004,6,25]],"date-time":"2004-06-25T18:08:09Z","timestamp":1088186889000},"page":"492-499","source":"Crossref","is-referenced-by-count":35,"title":["Carbohydrates and glycoproteins involved in bovine fertilization in vitro"],"prefix":"10.1002","volume":"68","author":[{"given":"S.","family":"Tanghe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann","family":"Van Soom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Duchateau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Nauwynck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"de Kruif","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,6,25]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(82)90124-0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1026"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1120230202"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/3.7.599"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)55872-5"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/3.10.827"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.18.6778"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod40.4.887"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(94)90152-X"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1093\/oxfordjournals.humrep.a136168","article-title":"A new predictive test for in\u2010vitro fertilization based on the induction of sperm acrosome reaction by N\u2010acetylglucosamine\u2010neoglycoprotein","volume":"10","author":"Brandelli A","year":"1995","journal-title":"Hum Reprod"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1996.tb01829.x"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)43402-8"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1995.tb01732.x"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199499000593"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod44.5.913"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1120150302"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod52.6.1395"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0930253"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1120210404"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1992.tb00342.x"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080390311"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/2.11.859"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/4.6.543"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.2307\/2529049"},{"key":"e_1_2_1_26_1","first-page":"170","volume-title":"Biotechnology in agriculture No. 11, laboratory production of cattle embryos","author":"Gordon I","year":"1994"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(199910)54:2<179::AID-MRD10>3.0.CO;2-J"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)56590-X"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1120050406"},{"key":"e_1_2_1_30_1","first-page":"1","volume-title":"The fallopian tubes. 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