{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:43:10Z","timestamp":1790876590407,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,9,29]],"date-time":"2004-09-29T00:00:00Z","timestamp":1096416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Zygote arrest 1 (Zar1) is an ovary\u2010specific maternal factor that plays an essential role during the oocyte\u2010to\u2010embryo transition in mouse. In this species, Zar1 expression is strictly limited to the oocyte, the zygote and, at a lower level, the 2\u2010cell embryo. Aim of the present study was to analyze the presence and the expression pattern of the Zar1 ortholog in bovine tissues and embryos. Reverse transcription (RT)\u2010polymerase chain reaction (PCR) analysis was performed in a panel of bovine tissues, in oocytes and pre\u2010implantation in vitro produced embryos. The results demonstrated that a Zar1 ortholog is present in cattle. In the adult, the gene is expressed in ovary, testis, muscle, and myocardium. The gene is also expressed in the oocyte, the zygote, and in all the stages of embryonic development until blastocyst formation. A semi\u2010quantitative RT\u2010PCR analysis revealed that Zar1 levels are constant through in vitro development with the exception of the 4\u2010cell stage, when a significant increase is observed. The exposure of fertilized oocytes to the RNA polymerase II inhibitor alpha\u2010amanitin was able to suppress this Zar1 increase indicating that transcription of this gene occurs at the 4\u2010cell stage. Zar1 is conserved in cattle but has an expression pattern different from the mouse. In particular, Zar1 expression in the adult is not limited to the ovary and in the embryo is expressed well beyond the oocyte to embryo transition. Moreover, the identification of Zar1 transcription at the 4\u2010cell stage represents the first characterization of one of the genes expressed in cattle embryos before the major onset of embryonic transcription. Mol. Reprod. Dev. 69: 375\u2013380, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20140","type":"journal-article","created":{"date-parts":[[2004,9,29]],"date-time":"2004-09-29T20:02:31Z","timestamp":1096488151000},"page":"375-380","source":"Crossref","is-referenced-by-count":34,"title":["Expression pattern of the maternal factor zygote arrest 1 (Zar1) in bovine tissues, oocytes, and embryos"],"prefix":"10.1002","volume":"69","author":[{"given":"Tiziana A.L.","family":"Brevini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Cillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Colleoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Lazzari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Galli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Gandolfi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,9,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080290205"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/35037669"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00482-4"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00486-1"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(03)00373-3"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)61779-9"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199400000861"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00480-0"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080260113"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0300493"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.8.6911"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/81547"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod60.5.1093"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(199905)53:1<8::AID-MRD2>3.0.CO;2-K"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.016022"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1079"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrd.20140","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.20140","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T20:32:06Z","timestamp":1700166726000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.20140"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,9,29]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,12]]}},"alternative-id":["10.1002\/mrd.20140"],"URL":"https:\/\/doi.org\/10.1002\/mrd.20140","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"value":"1040-452X","type":"print"},{"value":"1098-2795","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,9,29]]}}}