{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T05:07:38Z","timestamp":1767848858254,"version":"3.49.0"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,10,28]],"date-time":"2004-10-28T00:00:00Z","timestamp":1098921600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"German Research Foundation (Deutsche Forschungsgemeinschaft) (to ETP)","award":["To 114\/4"],"award-info":[{"award-number":["To 114\/4"]}]},{"name":"German Research Foundation (Deutsche Forschungsgemeinschaft) (to PM)","award":["M\u00fc 1017\/2"],"award-info":[{"award-number":["M\u00fc 1017\/2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Phospholipid\u2010binding proteins in the male genital tract are characterized by differing numbers Fn\u20102 modules (B\u2010domain) carrying N\u2010terminal extensions (A\u2010domain) of variable length. In the stallion, three different proteins were identified, SP\u20101, SP\u20102, and EQ\u201012. SP\u20101 and SP\u20102 of the AA'BB'\u2010 and ABB'\u2010type, respectively, are major proteins of the seminal plasma. Here we report the cDNA sequences of SP\u20101, and of a new member of the SP\u20102 family (SPnew) and the partial characterization of their iso\u2010 and glycoforms. The phosphorylcholine (PC)\u2010binding ability of the long Fn\u20102 protein, EQ\u201012, with four tandemly arranged Fn\u20102 modules was determined by PC\u2010affinity chromatography. Expression patterns of EQ\u201012, and the SP\u2010proteins were studied by means of RT\u2010PCR, Northern blot analysis and immunological approaches indicating differential expression along the male reproductive tract. The vast majority of the short SP\u20101 and SP\u20102 proteins are produced by the ampulla whereas EQ\u201012 originates from the epididymis. Indirect immunofluorescence microscopy of sperm isolated from different regions of the epididymis and Western blot analysis indicate that both, the long and the short Fn\u20102 proteins associate to the sperm surface during post\u2010testicular maturation. Sperm binding of Fn\u20102 proteins at the post\u2010acrosome and midpiece was at first detected in the corpus epididymis. Enhanced fluorescence intensity after ejaculation point to an increased number of molecules bound to the sperm surface. The function of these proteins is discussed in regard to their structure\u2013function relationships. Mol. Reprod. Dev. 70: 45\u201357, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20187","type":"journal-article","created":{"date-parts":[[2004,11,2]],"date-time":"2004-11-02T18:48:18Z","timestamp":1099421298000},"page":"45-57","source":"Crossref","is-referenced-by-count":37,"title":["Structural and molecular characterization of equine sperm\u2010binding fibronectin\u2010II module proteins"],"prefix":"10.1002","volume":"70","author":[{"given":"Mahnaz","family":"Ekhlasi\u2010Hundrieser","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bettina","family":"Sch\u00e4fer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christiane","family":"Kirchhoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Hess","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Bellair","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edda","family":"T\u00f6pfer\u2010Petersen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,28]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.023069"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00768-3"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3100615"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)00011-B"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(96)01310-5"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)00344-X"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00099-X"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0188-4409(01)00321-6"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"10149","DOI":"10.1016\/S0021-9258(19)50212-5","article-title":"Major proteins of bovine seminal plasma exhibit novel interactions with phospholipid","volume":"267","author":"Desnoyer L","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1993.1431"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1128"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(83)91078-1"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1997.00735.x"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1999.1593"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1160391"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)00448-6"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi010552"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi035647l"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.102.010827"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006960004440"},{"key":"e_1_2_1_22_1","unstructured":"HessO.1998.Localization and function of equine seminal plasma proteins HSP\u20101 and HSP\u20103. Thesis School of Veterinary Medicine Hannover."},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(83)91038-8"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/2.3.177"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080370203"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9888(199705)32:5<483::AID-JMS502>3.0.CO;2-J"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2410685"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0378(01)00098-5"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.10369"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-1981(99)00049-9"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-1981(99)00032-3"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002490050108"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00249-002-0234-2"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.137.10.4451"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(01)75869-9"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2795(200101)58:1<88::AID-MRD12>3.0.CO;2-D"},{"key":"e_1_2_1_38_1","first-page":"67","article-title":"Expression of CRISP proteins in the male equine genital tract","volume":"53","author":"Schambony A","year":"1998","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0531.2003.00403.x"},{"key":"e_1_2_1_40_1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.21836\/PEM20010208","article-title":"A.I.\u2010sperm of the stallion: Evaluation criteria and minimal standards\u2014a survey","volume":"2","author":"Sieme H","year":"2001","journal-title":"Pferdeheilkunde"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi000427i"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod52.6.1372"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod57.5.1080"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod59.4.768"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.3.590"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod65.1.41"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/5.4.314"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-010X(19991015)285:3<259::AID-JEZ9>3.0.CO;2-B"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1998.tb01163.x"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-1-39"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(02)00751-7"},{"key":"e_1_2_1_52_1","first-page":"189","volume-title":"The physiology of reproduction","author":"Yanagimachi R","year":"1994"}],"container-title":["Molecular Reproduction and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrd.20187","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrd.20187","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T18:40:58Z","timestamp":1700160058000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrd.20187"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,10,28]]},"references-count":51,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,1]]}},"alternative-id":["10.1002\/mrd.20187"],"URL":"https:\/\/doi.org\/10.1002\/mrd.20187","archive":["Portico"],"relation":{},"ISSN":["1040-452X","1098-2795"],"issn-type":[{"value":"1040-452X","type":"print"},{"value":"1098-2795","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,10,28]]}}}