{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:37:18Z","timestamp":1767854238913,"version":"3.49.0"},"reference-count":43,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2006,5,14]],"date-time":"2006-05-14T00:00:00Z","timestamp":1147564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The spermatogenic cell\u2010specific variant of glyceraldehyde 3\u2010phosphate dehydrogenase (GAPDS) has been cloned from a rat testis cDNA library and its pattern of expression determined. A 1,417 nucleotide cDNA has been found to encode an enzyme with substantial homology to mouse GAPDS (94% identity) and human GAPD2 (83% identity) isozymes. Northern blotting of rat tissue RNAs detected the 1.5 kb <jats:italic>Gapds<\/jats:italic> transcript in the testis and not in RNA from liver, spleen, epididymis, heart, skeletal muscle, brain, seminal vesicle, and kidney. The rat <jats:italic>Gapds<\/jats:italic> mRNA was first detected at day 29 of postnatal testis development, an age which coincides with the initial post\u2010meiotic differentiation of round spermatids. When isolated rat spermatogenic cell RNA was probed for <jats:italic>Gapds<\/jats:italic> expression, transcripts were detected only in round spermatids and condensing spermatids, but not in pachytene spermatocytes, demonstrating haploid expression of the <jats:italic>Gapds<\/jats:italic> gene. However, immunohistochemical staining of rat testis sections with anti\u2010GAPDS antisera did not detect GAPDS in round spermatids, but localized the protein only to stage XIII and later condensing spermatids as well as testicular spermatozoa, indicating that <jats:italic>Gapds<\/jats:italic> expression is translationally regulated. The current results are similar to those previously obtained for mouse GAPDS and human GAPD2, suggesting that reliable comparisons can be made between these species in toxicant screening and contraceptive development. Mol. Reprod. Dev. 1052\u20131060, 2006. Published 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrd.20235","type":"journal-article","created":{"date-parts":[[2006,5,15]],"date-time":"2006-05-15T06:25:41Z","timestamp":1147674341000},"page":"1052-1060","source":"Crossref","is-referenced-by-count":34,"title":["Expression of the spermatogenic cell\u2010specific glyceraldehyde 3\u2010phosphate dehydrogenase (GAPDS) in rat testis"],"prefix":"10.1002","volume":"73","author":[{"given":"Jeffrey E.","family":"Welch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randy R.","family":"Barbee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia L.","family":"Magyar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donna O.","family":"Bunch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah 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